Ungakhetha kanjani ipuleti le-ELISA elifanele?

Ungakhetha kanjani ipuleti le-ELISA elifanele?

Ukuma kwephansi
Iphansi eliphansi: Iphansi livundlile, libuye libizwe ngo-F phansi.Ukukhanya okudlula phansi ngeke kuchezuke, futhi ukudluliswa kokukhanya kungakhuliswa.Isetshenziselwa ukuhlolwa okudinga okuphansi okuzungezile ukuze kubonakale noma ezinye izizathu.
Izansi Eyindingilizi: Eyaziwa nangokuthi i-U-bottom, ihlinzeka ngokuhlanzeka okuphelele nokusebenza kokuxutshwa kwezinhlelo zokusebenza ezidinga ukuhlolwa kwezinsalela.
C-Phansi: Phakathi kwephansi eliyisicaba kanye nephansi eliyindilinga elinikeza imiphumela emihle yokuhlanza nokuhlanganisa izinzuzo zephansi eliyisicaba.
I-cone bottom: Eyaziwa nangokuthi i-V bottom, ifanele amasampula anembile kanye nokugcinwa kwamasampuli amancane ukuze athole kabusha amavolumu amancane.
Umbala
Iningi lama-ELISA likhetha amapuleti abonisa ngale njengento yokuhlola.Amapuleti amhlophe namnyama ngokuvamile asetshenziselwa ukutholwa kwe-luminescence.Amapuleti e-ELISA amnyama anomunca wawo wokukhanya, ngakho isiginali yawo ibuthakathaka kunamapuleti e-ELISA amhlophe.Amapuleti amnyama ngokuvamile asetshenziselwa ukubona ukukhanya okunamandla, njengokutholwa kwe-fluorescence;ngokuphambene nalokho, amapuleti amhlophe angasetshenziselwa ukubona ukukhanya okubuthakathaka, futhi avame ukusetshenziselwa ukuthuthukiswa kombala we-chemiluminescence kanye ne-substrate (isb. i-dual-luciferase reporter analysis gene).
Okubalulekile
Izinto ezijwayelekile ziyi-polyethylene, i-PE, i-polypropylene, i-PP, i-Polystyrene, i-PS, i-Polyvinylchloride, i-PVC, i-Polycarbonate, i-PC.
Izinto ezisetshenziswa kakhulu ku-ELSIA i-polystyrene ne-polyvinyl chloride.I-Polyvinyl chloride ithambile, izacile, iyasikwa futhi ayibizi.Okubi ukuthi ukuqedwa akukuhle njengamashidi e-polystyrene futhi phansi kwembobo akusiyo njengesicaba njenge-polystyrene.Nokho, kukhona ukukhuphuka okuhambisanayo kwamanani angemuva.Ngokuvamile, ingaphezulu lepuleti lokulebula le-enzyme kufanele liphathwe ngokuxhunyelelwa kwe-ionic, okwethula amaqembu asebenzayo asebenzayo njengeqembu le-aldehyde, iqembu le-amino kanye neqembu le-epoxy ebusweni bepolymer ukuze kuthuthukiswe ukusebenza kwendawo engaphansi.
Izindlela zokubopha ezihlukene
Ukubophezela okusebenzayo kwento ehlanganisiwe phansi