Amathuluzi avamile kanye nemishini kuma-cell laboratories Imikhumbi yeCultural

Amathuluzi ajwayelekile kanye nemishini kuma-cell laboratories Imikhumbi yeCultural,
Amaseli Amasiko Izitsha, lab petri izitsha, Petri Izitsha, izitsha zepulasitiki,

Namuhla ngizokwethula amathuluzi asetshenziswa kakhulu kanye nemishini kumalabhorethri we-cell culture, imikhumbi yamasiko, izindlela zokusebenzisa kanye nesingeniso esisebenzayo.
Uhlu lwemikhumbi yesiko elivamile kuma-cell laboratories.
Kunezinhlobo ezintathu eziyinhloko zemikhumbi yesiko elivamile kumalabhorethri amaseli, okuyilezi: imikhumbi yamasiko, imikhumbi ehlobene nokusebenza kwamasiko, nokunye okuhlinzekwayo.
1. Imikhumbi yamasiko
Ngokusho kwezinto ezibonakalayo, zihlukaniswe zibe izitsha zesiko lengilazi kanye nezitsha zesiko lepulasitiki.Phakathi kwabo, inzuzo yangaphambili ukuthi kulula ukukhula kweseli, kulula ukuyihlanza, futhi ingasetshenziswa ngokuphindaphindiwe.Inzuzo yalokhu yokugcina ukuthi ishibhile futhi ingasetshenziswa kanye.Njengamanje, kunezinhlobo ezintathu ezijwayelekile zemikhumbi yamasiko, okungukuthi: amabhodlela amasiko, izitsha ze-petri, namapuleti amasiko amaningi.
→ Ibhodlela lesiko: elenziwe ngengilazi noma ngepulasitiki, elisetshenziselwa ukulima nokusabalalisa amaseli.Ukucaciswa kwasekhaya kukhonjiswa ngomthamo (ml), kuyilapho imininingwane engenisiwe iboniswa ngendawo eyisisekelo (c㎡).
→ Izitsha zePetri: Zenziwe ngengilazi noma ngepulasitiki, zinezinto ezimbili ezinkulu ezisetshenziswayo.Enye ngeyokulayisha, ukuhlukanisa, nokucubungula izicubu;enye ingeye-cytotoxicity, ukuhlukaniswa kweseli elilodwa, nokuhlolwa kokufakwa kwe-isotopu.Ukucaciswa okujwayelekile ngu-10cm, 9cm, 6cm, 3.5cm, njll.
→ Ipuleti lezamasiko elinemithombo eminingi: elenziwe ngepulasitiki, lisetshenziselwa ukuhlola okuhlukahlukene kokutholwa, okufana nokuhlanganisa amaseli nokuhlolwa kwe-cytotoxicity.Ukucaciswa okuvame ukusetshenziswa yizimbobo eziyisi-6, izimbobo ezingama-24, izimbobo eziyi-12, izimbobo ezi-6, izimbobo ezi-4, njll.
2. Izitsha ezihlobene nokusebenza kwamasiko
Kunezinhlobo ezintathu eziyinhloko zezitsha ezihlobene nokusebenza kwesiko kuma-cell laboratories, okuyilezi: amabhodlela okugcina uketshezi, ama-pipette, nama-injection amasampula.
→ Ibhodlela lokugcina uketshezi: lisetshenziselwa ukugcina noma ukulungisa uketshezi oluphuzwayo, olufana noketshezi lwe-culture, i-serum nama-reagents.Ukucaciswa okuvame ukusetshenziswa yi-1000ml, 500ml, 250ml, 100ml, 50ml, 5ml, njll.
→ Izitsha: zihlukaniswa ngotshani obuthweswe iziqu kanye notshani obungenasikali.Owokuqala usetshenziselwa ukumunca nokudlulisa uketshezi, futhi imininingwane esetshenziswa kakhulu ngu-10ml, 5ml, 2ml, kanye no-1ml.Lesi sakamuva sisetshenziselwa ukumunca nokudlulisa uketshezi, i-pipet, ukuxuba kanye namaseli okudlula.
→ Umjovo oyisampula: owaziwa nangokuthi “i-pipettor”, esetshenziselwa ukumunca futhi kuhambise uketshezi noma ukuwisa amasampula.Engcono kakhulu i-micro-injector, enembile futhi elungele ukulangazelela, futhi engaqinisekisa okuqukethwe okunembile kwamasampuli okuhlola (noma ama-reagents) nokuphindaphinda okuhle.
3. Ezinye izinsiza
Ezinye izinto ezitholakala elabhorethri yamaseli zihlanganisa: amashubhu e-centrifuge (okuqoqa amaseli), amashubhu okuhlola (okubeka ama-reagents), iziqukathi zengilazi (zokugcina ama-pipette), amathangi okugcina (ukugcina izinto zamasiko amancane), ama-cryovials (ukuqandisa amaseli), kanye amasirinji ahlukahlukene, amabhekha, amasilinda okulinganisa, amafaneli, namalambu otshwala.

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