Amathiphu angu-3 wokukhetha izinto ezidlekayo zesiko lamaseli
1. Nquma imodi yokulima
Ngokwezindlela ezihlukene zokukhula, amaseli angahlukaniswa abe amaseli anamathelayo namaseli amisiwe, futhi kukhona namaseli angakhula abambelele noma amiswe okwesikhashana, njengamaseli e-SF9.Amaseli ahlukene futhi anezidingo ezihlukene zezinto ezisetshenziswayo zesiko lamaseli.Amaseli anamathelayo ngokuvamile asebenzisa izinto ezisebenzisekayo ezilashwe i-TC, kuyilapho amaseli amisiwe engenazo izidingo ezinjalo, kodwa izinto ezisebenzisekayo ezine-TC nazo zifanele ukukhula kwamaseli amisiwe.Ukukhetha izinto ezisebenzisekayo ezifanele, okokuqala, nquma imodi yesiko lamaseli ngokohlobo lweseli.
2. Khetha uhlobo olusebenzisekayo
Izinto ezisetshenziswayo ze-cell Culture zihlanganisa ipuleti le-cell culture, ibhodlela lesiko, isitsha samasiko, i-triangular shake flask, i-pipette, njll. Lezi zinto ezisetshenziswayo zinezici ezihlukene endaweni yamasiko, indlela yokusebenzisa, isakhiwo jikelele, njll. Ibhodlela lesiko lingelesiko elivaliwe, elingase ukunciphisa ukungcola;Ipuleti lempucuko kanye nesitsha samasiko kungokwesiko elivuleke kancane, elilungele ukuhlolwa kokulawula kanye nokuhlolwa kwe-gradient, kodwa futhi kulula ukuletha ukungcoliswa kwamagciwane, okudinga izidingo eziphakeme kubaqhubi.
Ngamafuphi, lapho sikhetha uhlobo lwezinto ezisetshenziswayo, kufanele sicabangele izidingo zokuhlola kanye nezintandokazi zokusebenza komuntu siqu.
3. Imininingwane ekhethiwe esebenzisekayo
Ukuhlolwa kwesiko lamaseli esilinganiso esikhulu kudinga okusebenzisekayo okunendawo yesiko elikhulu njengosekelo, kuyilapho izivivinyo ezincane zikhetha izinto ezisebenzisekayo ngendawo encane.Izimboni zamaseli zisetshenziselwa kakhulu isiko lamaseli amakhulu, njengokukhiqizwa kokugoma, i-monoclonal antibody, imboni yezemithi, njll;Ipuleti lesiko, isitsha samasiko kanye nebhodlela lesiko lifanele isiko lamaseli amancane elabhorethri;Ngaphezu kwesiko leseli lokumiswa, i-flask engunxantathu ingase isetshenziselwe ukulungisa, ukuxuba kanye nokugcinwa kwesiko lamasiko.Nquma ukucaciswa okuqondile kwezinto ezisebenzisekayo ngokwesilinganiso sosiko lweseli.
Izinto ezisetshenziswayo zesiko lamaseli ziyisisekelo sokuqinisekisa ukukhula okuhle kwamaseli, kanye nesihluthulelo sokusheshisa inqubo yokuhlola nokuqinisekisa umphumela wamasiko.Ukukhetha kufanele kucabangele izici ezifana nemodi yesiko lamaseli, isikali samasiko nezimo zaselabhorethri.
Vele, kubalulekile futhi ukukhetha inkundla enemikhiqizo ehlukahlukene, ukunikezwa kwezimpahla okuzinzile, ikhwalithi eqinisekisiwe kanye nezinsizakalo, njengeLabio, iLabio ingahlinzeka ngezinsizakalo zokuthenga eziphelele zokumisa okukodwa kwezinsiza zocwaningo lwesayensi zamalabhorethri emikhakheni yempilo yomhlaba. isayensi, imboni yezemithi, ukuvikelwa kwemvelo, ukuphepha kokudla, izikhungo zikahulumeni kanye nemithi yokwelapha.
Isikhathi sokuthumela: Feb-09-2023