Ishubhu le-centrifuge liyishubhu elilula elikwazi ukumelana nesivinini esiphezulu sokujikeleza nokucindezela, njengokuhlukanisa amasampula athile nokuhlukanisa intlenga enamandla amakhulu.Ishubhu ye-ultrafiltration centrifuge inezingxenye ezimbili ezifana neshubhu elingaphakathi neshubhu elingaphandle.Ishubhu elingaphakathi liwulwelwesi olunesisindo samangqamuzana athile.Phakathi ne-high speed centrifugation, lezo ezinesisindo se-molecular encane zizovuzela eshubhuni elingezansi (okungukuthi ishubhu langaphandle), futhi lezo ezinesisindo samangqamuzana esikhudlwana zizovaleleka eshubhuni eliphezulu (okungukuthi, ishubhu elingaphakathi).Lona umgomo we-ultrafiltration futhi uvame ukusetshenziselwa ukugxilisa amasampula.
Amashubhu e-Ultrafiltration centrifuge ngokuvamile angasetshenziswa ngaphandle kokwelashwa kusengaphambili, kodwa ekucubunguleni isampula yamaprotheni, ikakhulukazi izisombululo zamaprotheni ahlanjululwe (< 10ug / ml), izinga lokuthola kabusha lokugxilisa ingqondo ngolwelwesi lwe-ultrafiltration ngokuvamile alilona ubuningi.Nakuba izinto ze-PES zinciphisa ukukhangiswa okungaqondile, amanye amaprotheni, ikakhulukazi uma exubile, angase abe nezinkinga.Izinga lokubophezela okungaqondile liyahlukahluka ngokuya ngesakhiwo samaphrotheni ngamanye.Amaphrotheni aqukethe izizinda ezishajiwe noma ezine-hydrophobic cishe azobophezela ngendlela engenakuhlehliswa ezindaweni ezihlukene.I-passivation pretreatment ebusweni beshubhu ye-ultrafiltration centrifuge inganciphisa ukulahleka kwe-protein adsorption endaweni yolwelwesi.Ezimweni eziningi, ukulungiswa kwangaphambili kwekholomu ngaphambi kokugxilisa isisombululo seprotein encibilikayo kungathuthukisa izinga lokutakula, ngoba isixazululo singagcwalisa amasayithi angenalutho adsorption amaprotheni avezwe kulwelwesi nangaphezulu.Indlela ye-passivation ngaphambi kokucwilisa ikholomu ngevolumu ephezulu yesisombululo sokudlula isikhathi esingaphezu kwehora elingu-1, ugeze ikholomu kahle ngamanzi acwecwe, bese uyi-centrifuge kanye ngamanzi acwecwe ukuze ususe ngokuphelele isixazululo se-passivation esingase sihlale kufilimu. .Qaphela ukuthi ungavumeli ifilimu yome ngemva kokudlula.Uma ufuna ukulisebenzisa kamuva, udinga ukwengeza amanzi acwecwe oyinyumba ukuze ugcine ifilimu imanzi.
Amashubhu e-Ultrafiltration centrifuge ngokuvamile awakwazi ukuvalwa inzalo futhi aphinde asetshenziswe.Njengoba intengo ye-tube eyodwa ayishibhile, abantu abaningi bazama ukuphinda bayisebenzise - isipiliyoni ukuhlanza ubuso be-membrane ngamanzi a-distilled izikhathi eziningi futhi i-centrifuge kanye noma kabili.Ishubhu elincane elingafakwa i-centrifuged lihlehle lingacwiliswa emanzini acwengekile bese lifakwa i-centrifuged ngokuhlehla izikhathi eziningi, okuzoba ngcono.Ingasetshenziselwa isampula efanayo ngokuphindaphindiwe, futhi ingacwiliswa emanzini acwengekile uma ingasetshenziswa, kodwa ukungcola kwebhaktheriya kuzovinjelwa.Ungahlanganisi amasampuli ahlukene.Abanye abantu bathi ukucwiliswa ku-20% wotshwala kanye ne-1n NaOH (i-sodium hydroxide) kungavimbela ukukhula kwamagciwane futhi kuvimbele ukomiswa.Uma nje i-membrane ye-ultrafiltration ihlasela amanzi, ayikwazi ukuvunyelwa ukuba yome.Nokho, abanye bathi izobhidliza ulwelwesi lwesakhiwo.Kunoma yikuphi, abakhiqizi ngokuvamile abakusekeli ukusetshenziswa kabusha.Ukusetshenziswa okuphindaphindiwe kuzovimba usayizi wembotshana wolwelwesi lwesihlungi, futhi kubangele nokuvuza koketshezi, okuzothinta imiphumela yokuhlolwa.
Isikhathi sokuthumela: Sep-05-2022