Ukuhlukaniswa nobubanzi bokusetshenziswa kwepuleti lomthombo ojulile
Ukuhlukaniswa kwepuleti lomthombo ojulile:
1. Ngokwenani lezimbobo, ezivame kakhulu amapuleti angama-96 namapuleti angama-384.
2. Ngokusho kohlobo lwembobo, amapuleti angama-96 angahlukaniswa abe uhlobo lwembobo eyindilinga kanye nohlobo lwembobo yesikwele.Amapuleti emithombo angama-384 ayimigodi eyisikwele.
3. Ngokwesimo se-hole bottom, kukhona ikakhulukazi okumise okwe-U kanye no-V.
Ukuhlukaniswa kwepuleti le-deep orifice:
Iyini inhloso yepuleti lomthombo ojulile?
① Amasampula esitoreji:
Ingangena esikhundleni seshubhu le-centrifuge elivamile elingu-1.5ml ukuze ligcine amasampula, futhi libekwe ngobunono enqubweni yokugcina, lilondoloza indawo, elinomthamo omkhulu wokugcina, futhi lingakwazi ukumelana nesiqandisi esingu-80 ℃.Ibizwa nangokuthi ibhulokhi yokugcina.
② Ukwelashwa okuyisampula:
Ingasetshenziswa ngokuhambisana nezibhamu ezikhiphayo, amathuluzi okusebenza koketshezi oluningi oluzenzakalelayo nesofthiwe ukuze kuzuzwe ukusebenza komphumela ophezulu kumasampuli ebhayoloji, njengemvula yamaprotheni kanye nokukhipha uketshezi oluwuketshezi.Ukusebenza kahle kokucubungula isampula kuthuthukiswa kakhulu.Izinto ze-PP zingamelana nezinga lokushisa eliphezulu kanye nokwelashwa kokuvala inzalo ngomfutho ophezulu ongu-121 ℃.
③ Ukusebenza kwesampula:
Ivamise ukusetshenziswa kumasampula azenzakalelayo ahlukahlukene futhi ingafakwa ngqo ekamelweni lesampula lesampula elizenzakalelayo ukuze lijove.Uma kuqhathaniswa nebhodlela lomjovo lesampula lendabuko, alikwazi nje ukuphinda kabili inani lamasampula egumbini lesampula, kodwa futhi libone ukubekwa kwesampula.Ngemva kokucubungula ku-96 hole plate, isampula lingajovwa ngokuqondile ngaphandle kwanoma yimuphi umsebenzi oyisicefe.Dweba isampula emuva naphambili, beka isampula, uyimboze, faka ipulaki bese uhlanza ibhodlela.
Ipuleti le-Deep well ye-Labio lifaneleka kakhulu ukugcinwa kwesampula, ukuhlaziywa kwe-high throughput screening (HTS) okudinga i-motherboard, isiko lamaseli nethishu, ukuhlaziywa kwe-immunological kanye nezinye izinhlelo zokusebenza.Ipuleti linezimbobo ezingama-96 noma ezingama-384, linikeza umthamo ongu-0.5mL, 1.2mL, 2.0mL kanye no-2.2mL.Izimbobo zimakwe ngamaphethini ajwayelekile ezinhlamvu nezinombolo ukuze kube lula ukukhonjwa kwesampula futhi zingapakishwa ukuze zigcinwe.I-engeli ye-notch kulula ukuyithola kusampula yerobhothi kanye nesistimu yokubamba uketshezi oluzenzakalelayo.
Amapuleti e-polypropylene (PP) angeyona inyumba enziwe ngepropylene eluhlaza yekhwalithi ephezulu angakwazi uku-autoclaved futhi amelane namakhemikhali ahlukahlukene, ahlanganisa i-phenol, i-chloroform ne-DMS.Ukwengeza, ibhodi le-PP lingakwazi ukumelana namazinga okushisa aphansi - 80 ° C/- 112 ° F, okulenza libe lilungele ukusetshenziswa kwegumbi elibandayo nokugcinwa kwezinga lokushisa eliphansi.Khetha ibhodi le-polystyrene ukuze uthole ukucaca noma izakhiwo ezingaphezulu.
I-Labio 96 hole kanye namapuleti embobo angama-384 angasetshenziswa kanye ne-silicone gasket noma ifilimu yokuvala i-viscous ukuze kuncishiswe ukuhwamuka kwesampula kanye nokungcola.
Isikhathi sokuthumela: Nov-25-2022