Ukucaciswa okujwayelekile kweflask yesiko lamaseli

Ukucaciswa okujwayelekile kweflask yesiko lamaseli

I-cell culture isho indlela elingisa indawo yangaphakathi ku-vitro ukuze iyenze iphile, ikhule, ikhiqize futhi igcine ukwakheka nokusebenza kwayo okuyinhloko.Izinhlobonhlobo zezinto ezisetshenziswayo zesiko lamaseli ziyadingeka kusiko lweseli, lapho ibhodlela lesiko lamaseli lisetshenziswa kakhulu.

Ukuma kweflask yesiko leseli kuyisikwele futhi ibhodlela libanzi.Lo mklamo uwukwenza kube lula ukuvunwa kwamaseli.Uhlangothi lweflask luvamise ukuba nesithwathwa, okuyinto elungele u-opharetha ukuthi ayirekhode.Ngokuqondene nokucaciswa, izinto ezisetshenziswayo ezivamile ziyi-25cm2, 75cm2, 175cm2, 225cm2, njll. Sivame ukubhekisela kumthamo we-culture medium ibhodlela elingabamba.Kunezimo zohlelo lokusebenza ezahlukahlukene ngokuya ngemininingwane ehlukahlukene.

Amabhodlela amancane angama-25cm2 kanye no-75cm2 asetshenziselwa ukuvuselelwa kwamaseli kanye nokwandisa okuncane, ikakhulukazi esigabeni sokuqala sesiko lamaseli.Ingasetshenziswa futhi njengendawo yokugcina amangqamuzana abomvu egazi.Amabhodlela amakhulu angu-175 cm2 kanye no-225 cm2 asetshenziselwa ikakhulukazi isiko lamaseli esikalini esimaphakathi noma i-eukaryotic protein expression.I-protein expression ubuchwepheshe bebhayoloji obusebenzisa amagciwane, imvubelo, amaseli ezinambuzane, amaseli ezincelisayo noma amaseli ezitshalo ukuze aveze amaprotheni ofuzo angaphandle, futhi ingenye yezingxenye ezibalulekile zobuchwepheshe bobunjiniyela bofuzo.

I-cell culture flask isetshenziselwa ikakhulukazi isiko lamaseli anamathelayo.Nakuba ukucaciswa okuhlukile kwehluka ngokusetshenziswa, kufanele kuhlangabezane nezidingo zokukhula kwamaseli, njengokuthi i-DNase, ayikho i-enzyme ye-RNA, ayikho i-endotoxin, ayikho umthombo wesilwane, ukwelashwa kwe-surface TC, njll.

  I-Labio's cell culture flask inezici ezilandelayo:

1.Yenziwe nge-polystyrene(PS) yebanga lezokwelapha eseyintombi, enokucaca okuphezulu kanye nokumelana namakhemikhali okuhle

2.Ifaka indawo ebushelelezi kanye nedizayini yekepisi enogqinsi oluyiyunifomu, enesihlungi se-hydrophobic esingu-0.22 μm ukuze kushintshwe kalula

3.Intamo eyehlayo evumela ukusebenza okulula kwe-spatula yeseli nama-pipettors

4.Inendawo ebhaliwe eqhwa entanyeni, kanye neziqu ezinhlangothini zombili ze-ultrasonic welding esetshenziswa phakathi kwekhava neflask, ukugwema ukuvuza nensimbi ku-adhesive.

Idizayini engu-5.Stackable yokonga isikhala nokugcina kalula

6.Yenziwe egumbini elihlanzekile lebanga le-100,000, eline-DNase, i-RNase, i-pyrogen kanye ne-endotoxic

7.Ipakishwe ngayinye, inzalo ngokufakwa imisebe, SAL 10-6

8.Itholakala ngamakhono amathathu, i-TC iphathwa noma ayilashwa ongakhetha kukho.