Indlela yokusetshenziswa efanele kanye nezinyathelo ze-serological pipette

I-pipette ye-serological, eyaziwa nangokuthi i-pipette elahlayo, isetshenziselwa ukukala ngokunembile umthamo othile woketshezi, okufanele usetshenziswe kanye ne-pipette efanelekile.I-Pipette iyithuluzi lokulinganisa elisetshenziselwa ukudlulisa ngokunembile umthamo othile wesixazululo.I-pipette iyithuluzi lokulinganisa, elisetshenziselwa kuphela ukukala umthamo wesisombululo esiwukhiphayo.Kuyishubhu lengilazi elide nelincane elinesandiso esikhulu phakathi.Ukuphela kwayo okuphansi kusesimweni somlomo obukhali, futhi intamo yepayipi engenhla iqoshwe ngomugqa wokumaka, okuwuphawu lwevolumu eqondile okufanele ihanjiswe.

Indlela efanele yokusebenzisa kanye nezinyathelo ze-serum pipette:

1. Ngaphambi kokusetshenziswa: Uma usebenzisa i-pipette, qala ngokubheka uphawu lwe-pipette, izinga lokunemba, isikhundla somaka esikalini, njll.

2. Ukuphefumula: Bamba ingxenye engenhla ye-pipette ngesithupha nomunwe ophakathi wesandla sakho sokudla, bese ufaka umlomo ophansi we-pipette esixazululo esizomuncwa.Ukufakwa akufanele kube kuncane kakhulu noma kujule kakhulu, ngokuvamile kube ngu-10 ~ 20mm.Uma ingashoni kakhulu, izobangela ukumuncwa.I-Aspiration yesixazululo ebholeni lokugeza izindlebe izongcolisa isisombululo.Uma ijule kakhulu, izonamathela isixazululo esiningi kakhulu ngaphandle kweshubhu.Thatha ibhola lokugeza izindlebe ngesandla sobunxele, lixhume emlonyeni ongaphezulu weshubhu bese uhogela isixazululo kancane kancane.Okokuqala phefumula cishe 1/3 wevolumu yeshubhu.Cindezela umlomo weshubhu ngomunwe wokukhomba wesandla sokudla, uwukhiphe, uwubambe uvundlile, bese uzungezisa ishubhu ukuze wenze isisombululo sithinte ingxenye engaphezulu kwesikali ukuze sibuyisele amanzi odongeni lwangaphakathi.Bese ukhipha isisombululo emlonyeni ophansi we-tube bese uyilahla.Ngemuva kokugeza okuphindaphindiwe izikhathi ezintathu, ungakwazi ukumunca isisombululo cishe ngo-5mm ngaphezu kwesikali.Ngokushesha cindezela umlomo weshubhu ngomunwe wokukhomba wesandla sokudla.

3. Lungisa izinga le-liquid: phakamisa i-pipette phezulu futhi usuke ezingeni le-liquid, sula uketshezi odongeni olungaphandle lwe-pipette ngephepha lokuhlunga, ukuphela kweshubhu kuncike odongeni lwangaphakathi lwesitsha sesixazululo, ithubhu. umzimba uhlala umile, uphumule kancane umunwe wenkomba ukuze wenze isisombululo ku-tube sigeleze kancane siphuma emlonyeni ophansi, kuze kube yilapho phansi kwe-meniscus yesisombululo ku-tangent ekumaka, bese ucindezela ngokushesha umlomo wethubhu ngomunwe wenkomba.Susa i-liquid drop ngokumelene nodonga, uyisuse ku-pipette, futhi uyifake emkhunjini othola isisombululo.

4. Ukukhishwa kwesixazululo: Uma isitsha sokuthola ikhambi siyi-flask eyindilinga, i-conical flask kufanele itsheke ngo-30 °.I-pipette elahlayo kufanele ibe mpo.Umkhawulo ophansi we-tube kufanele ube eduze nodonga lwangaphakathi lwe-conical flask.Khulula umunwe wokukhomba bese uvumela isixazululo sigeleze kancane ngodonga lwebhodlela.Lapho izinga le-liquid liwela ekhanda lokukhipha, ithubhu ithinta udonga lwangaphakathi lwebhodlela cishe imizuzwana engu-15, bese isusa i-pipette.Isixazululo esincane esisele ekupheleni kwethubhu akufanele siphoqeleke ukuba siphume, Ngoba umthamo wesixazululo ogcinwe ekugcineni ucatshangelwe.