Umehluko phakathi kwe-cell culture flask ne-culture dish

I-cell culture iwubuchwepheshe bokuhlola obubaluleke kakhulu futhi isiphenduke indlela yocwaningo ebaluleke kakhulu emkhakheni we-biopharmaceutics, isayensi yezempilo, ukufakelwa kabusha komtholampilo, njll. Isiko lamaseli kufanele lithembele ezintweni ezisetshenziswa amangqamuzana ukuze kuzuzwe izimo ezidingekayo ekukhuleni kwamangqamuzana.Amabhodlela esiko lamaseli nezitsha zamasiko yizinhlobo ezimbili ezivamile.Uyini umehluko phakathi kwalezi zinto ezimbili ezisetshenziswayo?

Ibhodlela le-cell culture lifanele isiko lesikhathi eside kanye nokudlula njengamaseli embewu.Umlomo webhodlela mncane futhi amaseli akulula ukuthi angcoliswe.Izitsha zesiko lamaseli zifanele isiko lesikhashana ekuhlolweni okuhlukahlukene.Umehluko phakathi kwalokhu okubili usecicini lokuphepha kanye nenani lamaseli akhulisiwe.Isitsha sokuhlola samasiko esinamaseli njengesithwali noma into singcono, ngoba inani elisetshenzisiwe lincane, amaseli agcinwa, futhi isidlo samasiko silungele ukuhlolwa kokulawula, kodwa ukuvulwa kwesitsha sesiko kukhulu, okungaphezulu. kungenzeka ukuthi zingcoliswe, ngakho kufanele uqaphe kakhulu lapho usebenza.

I-culture flask isetshenziselwa isiko eliyinhloko le-tissue block noma isiko lamaseli angcoliswa kalula.Ngemuva kokuthi amaseli enziwe nge-subcultured, inganqunywa ngokuya ngokuthandwa komuntu.Indawo yebhodlela yesiko lamaseli inkulu, ngakho ibhodlela lesiko lingasetshenziswa lapho inani elikhulu lamaseli lidinga ukunwetshwa.

I-cell culture flask nezitsha zamasiko yizitsha ezisetshenziselwa amagciwane noma isiko lamaseli elabhorethri.Uhlobo oluthile lwezinto ezisetshenziswayo okufanele zisetshenziswe zincike ezidingweni ezithile zokuhlolwa, futhi futhi icabangela imodi yesiko lamaseli, noma ngabe isiko lokumiswa noma isiko lokubambelela.Izinto ezisetshenziswayo ezifanele ziyisisekelo sempumelelo yokuhlolwa.

Ukuze ufunde kabanzi mayelana nezinto ezisetshenziswayo ezihlolwayo, sicela ulandele iwebhusayithi yethu.I-Labio izoqhubeka nokuhlinzeka ngokubonisana kwakamuva kwezimpahla zokuhlola.