Umehluko phakathi kwekepisi elivaliwe kanye nekepisi eliphefumulayo lebhodlela lesiko leseli

Imvelo yesiko lamaseli ihlanganisa ubunyumba, izinga lokushisa elifanele (37~38 ℃), umfutho we-osmotic (260~320mmol/L), i-carbon dioxide kanye ne-PH efanele (7.2~7.4).Amabhodlela ayisikwele amaseli ngokuvamile adinga ukusebenzisa i-incubator noma i-greenhouse ukwenza isiko lamaseli.Ngokusho kwemvelo ehlukene yokusetshenziswa, izembozo zabo zihlukaniswe zibe izinhlobo ezimbili: ikhava evaliwe kanye nesembozo esiphefumulayo.

  Ikhava ephefumulayo: isembozo sinikezwa izimbobo zomoya, ezingavumela i-carbon dioxide endaweni ukuthi ingene ebhodleleni le-cell culture, idale izimo ezifanele zokukhula zokukhula kwamaseli.Kukhona ungqimba lwefilimu ephefumulayo oyinyumba phezulu kwekepisi yebhodlela, enemisebenzi emihle engangeni manzi futhi ephefumulayo.I-liquid ebhodleleni le-cell culture ngeke ithinte umgoqo we-microbial kanye nomphumela ophefumulayo wefilimu ephefumulayo ngemva kokuxhumana, engaqinisekisa ukukhula okuhle kwamaseli.

Amakepisi amabili ebhodlela lesikwele lesisiko seseli ahlangabezana nezidingo zezindawo zamasiko ezihlukene zokukhula kwamaseli.Lapho ukhetha ibhodlela lesikwele lesisiko seseli, khetha ikepisi elifanele ngokuya ngendawo ethile yesiko lamaseli ukugwema ukuthikameza ukukhula kwamaseli.