Kunezinhlobo ezimbili zamashubhu aqhwazayo: uhlobo lokuzungezisa kwangaphakathi kanye nohlobo lokuzungezisa lwangaphandle.Uyawazi umehluko phakathi kwabo?
Yikuphi okungcono?Ake sibheke.
I-screw cap yangaphakathi yeshubhu ye-cryopreservation isetshenziselwa ukufriza amasampula ebhayoloji nge-nitrogen ewuketshezi.I-silicone pad ku-nozzle ye-tube ithuthukisa ukuvala ithubhu ye-cryopreservation, engavimbela ngokuphumelelayo ukungena kwe-nitrogen ewuketshezi;
I-rotation cryopreservation tube yangaphandle isetshenziselwa ukufriza amasampula esiqandisini.Isikulufu sekepisi yokuzungezisa yangaphandle singanciphisa amathuba okungcola lapho uphatha amasampuli
Ukuzungezisa kwangaphandle kungenzeka kungcolise kakhulu kunokuzungeza kwangaphakathi, futhi akulula ukusebenza, kodwa futhi akunembile.Bheka ukuhlinzwa.Uketshezi oluzungezayo lwangaphakathi kulula ukungena kuntambo yekhava.Ngokuqondene nokuqhuma kweshubhu, ishubhu elizungezayo langaphakathi kulula ngempela ukuqhuma (kodwa futhi amathuba mancane kakhulu), ngakho-ke sicela usebenzise ngokuqaphela.
Manje ake sazi izici ze-screw cap yangaphakathi yeshubhu ye-cryopreservation
1. Ikepisi lesikulufu sangaphakathi seshubhu eliqandayo lidizayinelwe amasampuli aqandayo, futhi ikepisi eliluchungechunge lesikulufu sangaphandle linganciphisa amathuba okungcola lapho uphatha amasampula.
2. Ishubhu elishintshayo lokuqandisa elingaphakathi liklanyelwe ukuqanda amasampula esigabeni se-nitrogen ewuketshezi, futhi i-silica gel pad emlonyeni ithuthukisa ukuvaleka kweshubhu eliqandayo.
3. Umugqa we-cap cap kulula ukuzungeza ikhava.
4. Isigqoko sepayipi kanye nomzimba wepayipi kukhiqizwa ngezinto zokusetshenziswa kwe-PP ze-batch efanayo nemodeli, ngakho-ke i-coefficient yokwandisa efanayo iqinisekisa ukuthi ingavalwa kunoma yikuphi ukushisa.
5. Indawo enkulu yokumaka ilungele ukubhala.
6. Ishubhu lisobala ngokwedlulele futhi kulula ukulibona isampula.
7. Umklamo ophansi oyindilinga ulungele ukuthulula uketshezi nokunciphisa izinsalela.
Lawa amaphuzu olwazi mayelana nekepisi lesikulufu sangaphakathi seshubhu ye-cryopreservation.Ngethemba ukuthi okuqukethwe ngenhla kungakusiza.Siyabonga ngokubukela kwakho nokusekela.Ulwazi olwengeziwe luzolungiselelwa wena kamuva.Sicela unake isibuyekezo sewebhusayithi yethu.
Lawa amaphuzu olwazi mayelana nekepisi lesikulufu sangaphakathi seshubhu ye-cryopreservation.Ngethemba ukuthi okuqukethwe ngenhla kungakusiza.Siyabonga ngokubukela kwakho nokusekela.Ulwazi olwengeziwe luzolungiselelwa wena kamuva.Sicela unake isibuyekezo sewebhusayithi yethu.
Isikhathi sokuthumela: Sep-15-2022