Usufundile izinga lokuvala ifilimu?
Ini?Ubani omunye ongakwazi “ukuvala ifilimu”?Ngokushesha khathaza lesi sihloko ukuze sikufundise "ifilimu yokuvala uphawu" efanele!
Kunjalo, "ifilimu yokuvala" lapha iwukuvala ipuleti le-PCR elingu-96 ukuqinisekisa ukuthi ifilimu yokuvala ilingana eduze nepuleti lezimbobo ezingama-96 futhi ivimbele ukuhwamuka koketshezi, ukuze kuqinisekiswe ukuhlolwa okushelelayo.
1. Namathisela ifilimu yokuvala ebhodini
Khipha ulwelwesi olulodwa oluvala esikhwameni esizivalayo, bese uvala kabusha isikhwama esizivalayo ukuze ugcine i-enzyme ingenawo.Gcina umugqa ongezansi ubheke phezulu, ubambe ifilimu yokuvala, bese udiliza kancane umugqa ongezansi eduze komugqa we-tangent.
Bese, unamathisele umkhawulo owodwa we-adhesive surface yefilimu yokuvala uphawu ebhodini, futhi ubambe ibanga ne-engeli ukuze ugweme i-skew elandelayo.Enqubweni yokunamathisela, kunamathiselwe ingxenye eyodwa bese kuthi enye idonswe.
Ithiphu: Gqoka amagilavu njalo
● Uma kusetshenziswa ifilimu yokuvala yelebula elilodwa, susa umugqa kancane, qinisa ifilimu yokuvala ebhodini ukuze uyivale ebhodini lonke, bese uqhubeka nokukhipha umugqa.Le ndlela ingaqeda i-curl kanye ne-rollback ebangelwa ifilimu yokufaka uphawu.
● Uma kusetshenziswa umkhiqizo onamalebula amabili ekugcineni, hlubula umugqa ophakathi nendawo ngendlela eqhubekayo nebushelelezi.Ukukhumula ulayini kancane kancane kunciphisa ukugoqa.Qaphela ukuthi ungathinti indawo yokubopha ifilimu.
2. Ukucindezela ifilimu
Sebenzisa ipuleti lokucindezela ukuze ukhuhle kancane bese ucindezela ifilimu yepuleti lokuvala ukuze ulenze livaleke ngokuphelele epuletini.Uma kungekho laminates akhethekile, ungathola ikhadi elinemiphetho ebushelelezi, njengekhadi lasebhange noma ikhadi lebhasi.
Isinyathelo sokucindezela ifilimu sizokwenziwa okungenani kabili ngokuvundlile nangokuqondile.Kubalulekile ukusebenzisa amandla anele ukuze uthole uphawu oluhle.
Khwebula bese ucindezela ipuleti lokucindezela ulwelwesi eduze kwawo wonke amaphethelo angaphandle e-orifice plate okungenani kabili ukuze uqinisekise ukuthi kusetshenziswa ingcindezi eqinile neqhubekayo.Izimbobo nemiphetho kuzocindezelwa kanye.Ngemva kokuvala ifilimu yokuvala ngendlela efanele epuletini, khipha ingxenye ehlangene ngomugqa we-tangent.
Ithiphu: ● Uma ucindezela ifilimu, bamba ibhodi ngesinye isandla ukugwema ukunyakazisa ibhodi okunamandla.
3. Ukuhlola
Ngemuva kokuvalwa, hlola ngokucophelela ipuleti eliyisicaba ukuze uqinisekise ukuthi ifilimu ihlanganiswe eduze nepuleti.Qinisekisa ukuthi izimpawu zokunamathela eduze kwembobo ngayinye, yonke indawo engaphezulu yepuleti (okuhlanganisa nendawo ezungezile) ivaliwe, nokuthi ingabe kukhona uketshezi kulwelwesi.Ifilimu yokuvala uphawu akufanele ibe nemibimbi.Uma imibimbi ibonakala, ipuleti alivalwanga kahle.
● Kumapuleti ayisicaba anamachopho aphakanyisiwe, ukumiswa kwefilimu yokuvala epuleti kungase kungalungile, futhi ifilimu akufanele inabele phezulu odongeni oluseceleni lwepuleti.
Beka ipuleti elivaliwe okungenani imizuzu eyi-10 ngaphambi kokuqala ukuhlolwa kwe-PCR, futhi amandla okunamathela efilimu yokuvala azokwanda ngokuhamba kwesikhathi.Uma kungenzeka, sebenzisa i-centrifuge ekhethekile ye-orifice plate ukuze i-centrifugation.Ekugcineni, dlulisela ipuleti elivaliwe emshinini we-PCR ukuze uqale ukuhlola~
Ithiphu:
● Kumapuleti ayisicaba anamachopho aphakanyisiwe, ukumiswa kwefilimu yokuvala epuleti kungase kungalungile, futhi ifilimu akufanele inabele phezulu odongeni oluseceleni lwepuleti.
Beka ipuleti elivaliwe okungenani imizuzu eyi-10 ngaphambi kokuqala ukuhlolwa kwe-PCR, futhi amandla okunamathela efilimu yokuvala azokwanda ngokuhamba kwesikhathi.Uma kungenzeka, sebenzisa i-centrifuge ekhethekile ye-orifice plate ukuze i-centrifugation.Ekugcineni, dlulisela ipuleti elivaliwe emshinini we-PCR ukuze uqale ukuhlola~
Isikhathi sokuthumela: Dec-16-2022