Ungakhetha kanjani kahle ukusetshenziswa kwesiko lamaseli "amaflask, amapuleti nezitsha"?

Ungakhetha kanjani kahle ukusetshenziswa kwesiko lamaseli "amaflask, amapuleti nezitsha"?

Lapho kutshalwa amangqamuzana, ukuthi kufanele kusetshenziswe nini ama-flask amasiko kanye nesikhathi sokusebenzisa amapuleti emithombo kuncike enjongweni nasezidingweni zokuhlolwa.Ngokuvamile, ama-flask amasiko eseli asetshenziselwa isiko lamaseli ayisisekelo kanye ne-subculture evamile, futhi inani elikhulu lamaseli okuhlola lingatholakala.

Amaflask okukhiqiza amaseli enziwe ngezinto ezisezingeni eliphezulu ze-polystyrene (PS), akhiqizwa ngokubumba okunembe kakhulu kanye nezinqubo zokukhiqiza ezizenzakalelayo ngokuphelele.Imikhiqizo isetshenziselwa isiko lamaseli elabhorethri.Izakhiwo zabo ezinhle kakhulu ze-optical zisiza ukubonwa kwe-microscopic.Ingaphezulu liphathwe nge-TC ukuze kuqinisekiswe ukunamathela kweseli.Imiphumela engcono.

1) Ungawakhetha kanjani amaflaski amasiko namapuleti amasiko kumaseli amasiko

Okokuqala, khetha ngokusekelwe ekukhiqizeni kwamaseli okulindelekile.

Okwesibili, khetha ngokusekelwe ekwazisweni kwemisebenzi yokuhlola.Kungakhathaliseki ukuthi ishintsha imidiya, i-passage, noma amaseli okuvuna, ukusebenza kwezitsha zamasiko kulula kakhulu, kodwa ngenxa yokuvuleka kwayo okukhulu, kulula ukungcolisa.

2) Kungcono ukusebenzisa amapuleti e-cell culture ocwaningweni olusebenzisa amaseli njengabathwali noma izinto, njengokuhlola ukuthambekela kwezidakamizwa, i-MTT (i-96-well culture plate), i-immunohistochemistry (i-6-well culture plate), njll.