Iyini ifilimu yokuvala uphawu?
Ifilimu yokuvala ipuleti iyifilimu esobala yokuvala ipuleti esebenzisa ijeli, esetshenziswa kabanzi ekuhlolweni ngamapuleti angama-96/384, njenge-PCR, qPCR, ELISA, isiko lamaseli, isitoreji sesikhathi eside, ukucutshungulwa kwendawo yokusebenza okuzenzakalelayo, cishe nakho konke ukuhlola. .Umsebenzi wayo oyinhloko ukuqinisekisa ukuthi ifilimu yokuvala inamathele eduze kwepuleti lomthombo we-96 / 384 ukuvimbela ukuhwamuka kwamanzi.
Ngokunokwenzeka, izingane ezivame ukwenza lokhu kuhlola ziye zahlangabezana nezinkinga ezihlukahlukene ezifana ne-edge warping, ukuhwamuka, nokuklebhuka.Imikhiqizo okunzima ukuyikhulisa ihwamuke phakathi!inhliziyo yomuntu injengomlotha ofileyo, ichithekile ngokuphelele.
Uma ufuna ukwenza umsebenzi omuhle, kufanele uqale ulole amathuluzi akho.Akulula ukuthola izimo zokuhlola, thenga ipuleti le-PCR elisebenziseka kalula futhi elingaguquguquki, kanye nefilimu yokuvala ipuleti elibonisa ngale.Sidinga futhi ukuqonda indlela yokunamathisela ifilimu efanele!
Indlela efanele yokufaka isicelo sefilimu imi kanje:
Khipha ifilimu yokuvalwa kwepuleti elilodwa noma i-aluminium evala ipuleti esikhwameni esikhwameni esizivalayo, bese usivala kabusha isikhwama esizivalayo ukuze ulondoloze indawo engenaso i-enzyme.
Bamba ifilimu yokuvala noma i-aluminium foil yokuvala indawo engemuva ibheke phezulu.
Songa ilebula yokugcina phansi endaweni esekelayo.
▪ Uma umkhiqizo osetshenziswayo kuyifilimu yokuvala noma i-aluminium foil yelebula elisesiphelweni esisodwa, khipha ingxenye yephepha eliwusekelayo, bese ugxilisa ifilimu yokuvala noma i-aluminium foil ebhodini ukuze uyivale ebhodini lonke, bese uqhubeka nokukhipha iphepha lokusekela.Le ndlela ingaqeda i-curl kanye ne-rollback ebangelwa ifilimu yokuvala uphawu noma i-aluminium foil.
▪ Uma usebenzisa umkhiqizo onamalebula amabili ekugcineni, hlubula umugqa ophakathi nendawo ngendlela eqhubekayo futhi ebushelelezi.Khipha kancane i-liner ukuze unciphise ama-curl.Qaphela ukuthi ungathinti indawo yokubopha ifilimu.
Bamba izingxenye ezimhlophe kuzo zombili iziphetho ngezandla zombili bese wehlisela i-diaphragm ku-orifice plate.
Khipha kancane bese uvala ifilimu yepuleti noma i-aluminium foil ngefilimu yokucindezela ipuleti ukuze uyivale epuletini.Lesi sinyathelo kufanele senziwe okungenani kabili ngokuvundlile nangokuqondile.Ukusebenzisa amandla anele kubalulekile ukuze uthole uphawu oluhle.(bheka umdwebo wohlelo lwendlela yokuvala ngezansi):
▪ Khwebula futhi ucindezele ipuleti okungenani kabili kuwo wonke amaphethelo angaphandle e-orifice plate ukuze uqinisekise ingcindezi eqinile futhi eqhubekayo.
▪ Ngemva kokuvala, hlola ipuleti eliyisicaba ukuze uqinisekise ukuthi ifilimu/i-foil iboshelwe ngokuqinile epuletini.Ifilimu yokuvala uphawu noma i-aluminium foil akufanele ibe nemibimbi.Uma imibimbi ibonakala, kubonisa ukuthi ipuleti alivalwanga kahle.Kufanele futhi kuqashelwe ukuthi ifilimu yokuvala uphawu noma ucwecwe lwe-aluminium akufanele lukhuphukele phezulu odongeni oluseceleni lwepuleti.Kumapuleti ayisicaba anemiphetho ephakanyisiwe, lokhu kungenzeka ngenxa yokuthi ifilimu elivalayo noma i-aluminium foil ayimi kahle epuletini, noma amalunga emikhawulweni yomibili awadatshuliwe.Qinisekisa izimpawu zokunamathisela embobeni ngayinye, futhi yonke indawo yepuleti (okuhlanganisa ne-periphery) ivaliwe.
Ngemva kokuba ifilimu elivalekayo noma i-aluminium foil ivalwe kahle ebhodini, dabula ilunga elimhlophe kuzo zombili iziphetho eduze kwe-tangent.(umphumela uboniswa esithombeni esingezansi):
▪ Kungcono ukushiya ipuleti elivaliwe okungenani imizuzu eyi-10 ngaphambi kokuqala ukuhlola kwe-PCR, futhi ukunamathela kwefilimu yokuvala kuzokwanda ngokuhamba kwesikhathi.
▪ Dlulisela ipuleti emshinini we-PCR bese usebenzisa umshini we-PCR.
Kunezinhlobo eziningi zamafilimu okuvalwa kwamapuleti e-Labio, anganikeza cishe zonke izinhlobo zamafilimu okuvalwa kwepuleti ukuze afake izicelo zokuhlola zabasebenzisi, futhi angasetshenziswa ezinhlelweni eziningi ezihlanganisa i-PCR, qPCR, ELISA, isiko lamaseli, isitoreji sesikhathi eside, okuzenzakalelayo. ukucubungula indawo yokusebenza, njll.
Isikhathi sokuthumela: Sep-08-2022