Ungakhetha kanjani "i-tube yokuqandisa" enhle kakhulu?
Ishubhu le-cryo elisebenziseka kalula alikwazi ukuhlangabezana nezidingo zokuhlola kuphela, kodwa futhi linciphisa amathuba ezingozi zokuhlola ngezinga elithile.
Namuhla sizosebenzisa izindlela ezi-3 ukukhetha i-cryo tube.
Isinyathelo sokuqala: impahla
Njengoba sonke sazi, amashubhu okuqandisa asetshenziselwa kakhulu ukuthutha okunezinga lokushisa eliphansi kanye nokugcinwa kwamasampula ezicubu noma amaseli, ngokuvamile ocwaningweni lwebhayoloji kanye nezinkambu zezokwelapha.
Ngenxa yokuthi ishubhu eliqandayo lixhumene ngqo nesampula, isinyathelo sokuqala ukukhetha into efanele ukuze ugweme ukungcoliswa kwesampula.
Ngokuvamile, amashubhu okuqandisa enziwe ngezinto ngaphandle cytotoxicity.Izinto ezisetshenziswa kakhulu kulabhorethri ipulasitiki nengilazi.Kodwa-ke, ngenxa yokuthi ama-cryotubes engilazi awakwazi ukusetshenziswa kuma-centrifuges anesivinini esikhulu noma ahamba ngesivinini esikhulu, ama-cryotube epulasitiki avame ukusetshenziswa.
Kunezinto eziningi zepulasitiki, ungakhetha kanjani?
Amagama amahlanu, "i-polypropylene material" Khetha ngokuzethemba!
I-Polypropylene inamakhemikhali amahle kakhulu nokuzinza kwezinga lokushisa.Ngaphansi kwesimo segesi se-nitrogen ewuketshezi, ingamelana nezinga lokushisa eliphansi ukuya ku-187 ℃.
Ukwengeza, uma izidingo zokuphepha kwesampula ziphakeme kakhulu, izinto ezingezona ze-mutagenic kanye namashubhu ahambisanayo e-VID angenayo i-pyrogen angakhethwa.Futhi sicela ungayivuli ngaphambi kokuyisebenzisa.Uma isivele ivuliwe, kumele ivalwe inzalo ngaphambi kokusetshenziswa!
Isinyathelo sesibili: Ukubunjwa
Ishubhu lokuqandisa ngokuvamile lakhiwa isivalo seshubhu kanye nomzimba weshubhu, ohlukaniswa ube yithubhu yokuqandisa ikepisi yangaphakathi kanye neshubhu yokuqhwaza yangaphandle.Uma isampula izogcinwa esigabeni se-nitrogen ewuketshezi, sebenzisa ishubhu lokuqandisa elijikelezayo langaphakathi elinephedi yejeli ye-silica;Uma isampula izogcinwa ezintweni ezisebenza ngomshini, njengesiqandisi, kusetshenziswa ishubhu yokuqandisa ezungezayo yangaphandle, ngokuvamile ngaphandle kwephedi yejeli ye-silica.
Ngezwi elilodwa:
Sekukonke, ukumelana nezinga lokushisa okuphansi kweshubhu lokuphotha elingaphakathi le-cryopreservation kungcono kunokweshubhu lokuqandisa lokuphotha langaphandle, okufanele likhethwe ngokuya ngezidingo zangempela.
Isinyathelo sesithathu: imininingwane
Ngokwezidingo zokuhlola, amashubhu e-cryopreservation ngokuvamile anemininingwane engu-0.5ml, 1.0ml, 2.0ml, 5ml, njll.
Ishubhu yokuqandisa yesampula yebhayoloji evame ukusetshenziswa ngokuvamile ingu-2ml ngosayizi.Kufanele kuqashelwe ukuthi umthamo wesampula ngokuvamile awukwazi ukudlula izingxenye ezimbili kwezintathu zevolumu yeshubhu eliqandisayo.Ngakho-ke, ishubhu eliqandayo elifanele kufanele likhethwe ngokuya ngosayizi wesampula eqandisiwe
Ngaphezu kwalokho, kunomehluko phakathi kwe-double layer kanye ne-non-double layer, ingasungulwa futhi ayikwazi ukusungulwa, yasekhaya kanye nokungeniswa, kanye nentengo.Lezi yizici okufanele zicatshangelwe lapho kukhethwa ishubhu lokuqandisa.
Isikhathi sokuthumela: Dec-16-2022