Ungakhetha kanjani ipuleti le-PCR lezinto ezisetshenziswa elabhorethri?
Amapuleti e-PCR ngokuvamile anembobo engu-96 kanye nezimbobo ezingu-384, alandelwe yimigodi engu-24 kanye nezimbobo ezingu-48.Ithuluzi le-PCR elisetshenzisiwe kanye nemvelo yohlelo lokusebenza oluqhubekayo izonquma ukuthi ibhodi le-PCR likulungele yini ukuhlola kwakho.Ngakho-ke, ungakhetha kanjani ibhodi le-PCR lezinto ezisetshenziswayo zaselabhorethri ngendlela efanele?
I-1, Izinhlobo ezihlukene zesiketi azinawo amabhodi esiketi futhi azinawo amaphaneli azungezile.
Lolu hlobo lwepuleti lokusabela lungajwayelaniswa namamojula amaningi ezinsimbi ze-PCR nezinsimbi zesikhathi sangempela ze-PCR, kodwa alufanele ukusetshenziswa okuzenzakalelayo.
I-half-skirt plate inemiphetho emifushane ezungeze umkhawulo wepuleti futhi inikeza ukusekelwa okwanele ngesikhathi sokudlulisa uketshezi.Iningi lamathuluzi e-Applied Biosystems PCR asebenzisa amapuleti anesiketi esiyingxenye.
Ibhodi le-PCR elinesiketi esigcwele linephaneli yonqenqema elimboza ubude bebhodi.Lolu hlobo lwebhodi lufanele ithuluzi le-PCR elinemojula egqamile (evumela ukusebenza okuzenzakalelayo), futhi ingashintshwa ngokuphepha nangokuzinza.Isiketi esigcwele siphinde sithuthukise amandla emishini, yenze ifaneleke kakhulu ukusebenzisa inkundla yerobhothi ekuhambeni komsebenzi okuzenzakalelayo.
2. Izinhlobo zamaphaneli ahlukene
Idizayini yephaneli eyisicaba egcwele isebenza kumathuluzi amaningi e-PCR futhi ilungele ukuvalwa nokucutshungulwa.
Umklamo wepuleti le-convex onqenqemeni unokuvumelana nezimo okuhle kwamanye amathuluzi e-PCR (njenge-Applied Biosystems PCR instrument), esiza ukulinganisela ukucindezela kwesivalo sokushisa ngaphandle kwesidingo se-adaptha, ukuqinisekisa ukudluliswa kokushisa okuhle nemiphumela yokuhlola enokwethenjelwa.
Amapuleti e-PCR ngokuvamile angahlinzeka ngezinhlobonhlobo zemibala ehlukene ukuze kube lula ukuhlukaniswa okubonakalayo nokuhlonza amasampuli, ikakhulukazi ekuhlolweni komphumela ophezulu.Nakuba umbala wepulasitiki ungenawo umthelela ekwandiseni i-DNA, kunconyelwa kakhulu ukusebenzisa izinto ezidlekayo zepulasitiki ezimhlophe noma izinto ezidlekayo zepulasitiki eziyisithwathwa kunezinto ezisebenzisekayo ezibonisa ngale lapho kusethwa ukusabela kwe-PCR yobuningi besikhathi sangempela se-fluorescence ukuze kuzuzwe ukutholwa kwe-fluorescence ebucayi.
4. Izikhundla ezahlukene ze-chamfer
Ukusikwa kwekhona kuyikhona elingekho lepuleti le-PCR, elincike entweni ezoshintshwa.I-chamfer ingase ibe ku-H1, H12 noma ku-A12 yepuleti le-96-hole, noma i-A24 yepuleti le-384-hole.
5, ifomethi ye-ANSI/SBS
Ukuze ihambisane nezinhlelo ezahlukene zokuphatha uketshezi oluzihambelayo olusebenzayo, ibhodi le-PCR kufanele lithobele i-American National Standards Association (ANSI) kanye ne-Society for Biological and Molecular Sciences (SBS), manje engaphansi kwe-Laboratory Automation futhi Screening Association (SLAS).Ibhodi elihambisana ne-ANSI/SBS linosayizi ojwayelekile, ubude, indawo yembobo, njll., okuwusizo ekucubunguleni okuzenzakalelayo.
6. Unqenqema lwembobo
Kukhona umphetho ophakanyisiwe ozungeze umgodi.Lo mklamo ungasiza ukuvala ngefilimu yepuleti lokuvala ukuvimbela ukuhwamuka.
7, Mark
Ngokuvamile kuwuphawu oluphakanyisiwe lwe-alphanumeric olunombhalo wesandla omhlophe noma omnyama ngombala oyinhloko ukuze ubukeke kalula.
Isikhathi sokuthumela: Feb-10-2023