Isetshenziswa kanjani ishubhu le-cryopreservation ngokwesayensi nangendlela efanele
Ukusetshenziswa kweshubhu le-cryopreservation kuyisayensi, akuyona i-trilogy elula njengokuvula ithangi le-nitrogen eliwuketshezi, ukulifaka eshubhu le-cryopreservation nokuvala ithangi le-nitrogen eliwuketshezi.Ukusetshenziswa okungokwesayensi nokulungile kwamashubhu e-cryopreservation kungagwema ukulahlekelwa amasampula futhi kuvikele ukuphepha kwabahloli.
Ukusetshenziswa kweshubhu le-cryopreservation kuyisayensi, akuyona i-trilogy elula njengokuvula ithangi le-nitrogen eliwuketshezi, ukulifaka eshubhu le-cryopreservation nokuvala ithangi le-nitrogen eliwuketshezi.Ukusetshenziswa okungokwesayensi nokulungile kwamashubhu e-cryopreservation kungagwema ukulahlekelwa amasampula futhi kuvikele ukuphepha kwabahloli.
Ishubhu eqandayo: izinyathelo zokuqandisa
Geza amaseli ngesixazululo se-PBS esishisiwe, ncela isisombululo, futhi umboze amaseli ngesisombululo esiqukethe i-trypsin ne-EDTA (ungqimba oluwuketshezi oluncane lwanele, futhi ukugcwala kwe-trypsin ne-EDTA kudinga ukunqunywa ngokuya ngomugqa weseli).
Fukamisa amaseli ku-37 ℃ imizuzu emi-3-5.
Ngemuva kokuthi amangqamuzana ahlukane phansi, ukucwiliswa kuqediwe, i-serum equkethe i-medium yengezwa, futhi amaseli amiswe ngobumnene nge-pipette.
I-Centrifuge ukumiswa kweseli (500 xg, 5 min) nokumiswa kabusha okuphakathi okuqukethe i-serum.
Isibalo samaseli.
I-Centrifuge ukumiswa kweseli (500 xg, imizuzu emi-5), khipha amandla amakhulu, bese umisa kabusha amaseli ngeserum equkethe okuphakathi kwevolumu efanelekile.
Hlanganisa amaseli kanye nesisombululo se-cryopreservation (60% medium, 20% fetal bovine serum, 20% DMSO) ngesilinganiso sevolumu esingu-1:1, bese uwadlulisela ku-Cryo STM cryopreservation tube.Ukuminyana kwamaseli aqandisiwe 1-5 × 106 izingcezu/ml.
I-Cryo equkethe amaseli I-STM cryopreservation tube inconywa ukuthi iphole ngenani elingu-− 1 K/min, futhi ishubhu le-cryopreservation lingafakwa esitsheni esiqukethe isopropanol ku-− 70 ℃.Uma i-Cryo STM cryopreservation tube igcina amanye amasampula, angabekwa ngokuqondile ku-− 20 ℃, − 70 ℃ noma esigabeni segesi se-nitrogen ewuketshezi.Ukuqinisekisa ukuthi isampula liqandiswe ngendlela efanayo, 4 mL kanye no-5 mL Cryo Ishubhu ye-sTM cryopreservation idinga ukufakwa esiqandisini ngo-− 20 ℃ ubusuku bonke, bese idluliselwa esigabeni segesi esingu-− 70 ℃ noma i-nitrogen ewuketshezi.
Bese udlulisela ishubhu ye-Cryo.sTM cryopreservation ethangini le-nitrogen eliwuketshezi.Ukuze ugweme ukungcola (okufana ne-mycoplasma) nokucatshangelwa kokuphepha, sicela ubeke i-Cryo.sTM cryopreservation tube esigabeni segesi se-nitrogen enguketshezi, hhayi esigabeni soketshezi.
Ungayisebenzisa kanjani i-cryopreservation tube ngokwesayensi nangendlela efanele?Inkampani yethu yakhiwe ochwepheshe abanesizinda semboni kanye nolwazi lwemakethe olucebile ukuze banikeze imikhiqizo yenkundla yocwaningo lwesayensi yezempilo kanye nezinsizakalo zabacwaningi besayensi.Ngeke nje ihlangabezane nezidingo ezikhethekile zamakhasimende e-R&D ezinhlotsheni zemikhiqizo nokupakishwa, kodwa futhi ihlangabezane nezidingo ezibanzi zamabhizinisi okukhiqiza kuzo zonke izigaba kusukela esikalini esincane, esikalini esimaphakathi kuya ekukhiqizeni ngezinga elikhulu.
Isikhathi sokuthumela: Nov-25-2022