Ukuhlukaniswa kwezitsha zePetri——
1. Ngokusho kokusetshenziswa okuhlukene kwezitsha zesiko, zingahlukaniswa zibe izitsha zesiko lamaseli kanye nezitsha zesiko lebhaktheriya.
2. Ingahlukaniswa ibe yizitsha ze-petri zepulasitiki kanye nezitsha ze-petri zengilazi ngokusho kwezinto ezihlukene zokukhiqiza, kodwa izitsha eziningi ze-petri ezingenisiwe kanye nezitsha ze-petri ezilahlwayo ziyizinto zepulasitiki.
3. Ngobukhulu obuhlukahlukene, zingahlukaniswa ngokujwayelekile zibe ngu-35mm, 60mm no-90mm ububanzi.150mm Petri dish.
4. Ngokomehluko wokuhlukaniswa, ingahlukaniswa ngezitsha ezi-2 ezihlukene zePetri, izitsha ezi-3 ezihlukene zePetri, njll.
5. Izinto zezitsha zesiko zihlukaniswa ngokuyisisekelo zibe izigaba ezimbili, ikakhulukazi ipulasitiki nengilazi.Ingilazi ingasetshenziselwa izinto zezitshalo, isiko le-microbial, kanye nesiko lokunamathela lamaseli ezilwane.Izinto zepulasitiki zingase zibe izinto ze-polyethylene, ezingasetshenziswa kanye noma izikhathi eziningi.Zilungele ukujova elabhorethri, ukujova, kanye nemisebenzi yokuhlukanisa amagciwane, futhi zingasetshenziselwa ukutshala izinto zezitshalo.
Kungani isitsha sePetri sibheke phansi kusiko lwe-lithographic——
1. Ngesikhathi sokusebenza, kungase kube namaconsi amanzi noma amagciwane esembozweni se-petri dish.Isiko elibheke phansi lingavimbela amaconsi amanzi noma ama-microorganisms esembozweni ukuthi angawi esitsheni se-petri.
2. Phakathi nenqubo yesiko, amabhaktheriya azokhiqiza amanye ama-metabolites ayingozi ekukhuleni nasekukhiqizeni amabhaktheriya ngesikhathi sokukhiqizwa kwe-metabolic, ukukhulula ukushisa nokukhipha amanzi.Uma amabhaktheriya engakhuliswanga phansi, amaconsi amanzi azowela endaweni yesiko, okuthinta ukukhula kwamakoloni.
3. Uma inhloso yesiko iwukuqoqa ama-metabolite ebhaktheriya, futhi ama-metabolites ancibilika kalula emanzini, isiko elihlanekezelwe lingenza kube lula ukuqoqwa.
Ngesikhathi sesiko, kuzoba nomhwamuko omningi esitsheni samasiko, futhi ukufingqa komhwamuko wamanzi esembozweni sesitsha kuzokhiqiza amaconsi amanzi.Uma isidlo samasiko sibekwe endaweni efanele, amaconsi amanzi azohlakaza amakoloni.Ngale ndlela, ikoloni elikhulu lingase lihlakazeke libe amakoloni amaningi amancane, okubangela inkinga enkulu ekutshalweni nokubala kwamagciwane.Uma kubangelwa, i-culture medium iphezulu futhi isidlo singaphansi kwesembozo, futhi amaconsi amanzi ngeke awele ekoloni.
Izindlela zokuphepha zokusetshenziswa kwezitsha zePetri——
1. Ngemuva kokuhlanza nokukhipha amagciwane ngaphambi kokusetshenziswa, ukuhlanzeka kwezitsha zamasiko kunomthelela omkhulu emsebenzini, okungathinta i-pH yesiko lesiko.Uma kukhona amakhemikhali athile, azovimbela ukukhula kwamagciwane.
2. Izitsha ezisanda kuthengwa zamasiko kufanele zigezwe ngamanzi ashisayo kuqala, bese zicwiliswa ku-1% noma 2% isixazululo se-hydrochloric acid amahora amaningana ukuze kukhishwe izinto zamahhala ze-alkaline, bese zihlanzwa kabili ngamanzi a-distilled.
3. Ukuze uhlakulele amagciwane, sebenzisa umusi we-high pressure (ngokuvamile i-6.8 * 10 Pa high pressure steam ukuya emandleni esihlanu), yifake inzalo imizuzu engu-30 ku-120 ℃, uyomise ekamelweni lokushisa, noma usebenzise ukushisa okomile ukuvala inzalo, okungukuthi, ukubeka. isidlo samasiko kuhhavini, lawula izinga lokushisa ku-120 ℃ amahora angu-2, bese ubulala amazinyo e-bacterial.
4. Izitsha ezifakwe inzalo kuphela ezingasetshenziselwa ukujova nokutshala.
Kungani isitsha sePetri sibheke phansi kusiko lwe-lithographic——
1. Ngesikhathi sokusebenza, kungase kube namaconsi amanzi noma amagciwane esembozweni se-petri dish.Isiko elibheke phansi lingavimbela amaconsi amanzi noma ama-microorganisms esembozweni ukuthi angawi esitsheni se-petri.
2. Phakathi nenqubo yesiko, amabhaktheriya azokhiqiza amanye ama-metabolites ayingozi ekukhuleni nasekukhiqizeni amabhaktheriya ngesikhathi sokukhiqizwa kwe-metabolic, ukukhulula ukushisa nokukhipha amanzi.Uma amabhaktheriya engakhuliswanga phansi, amaconsi amanzi azowela endaweni yesiko, okuthinta ukukhula kwamakoloni.
3. Uma inhloso yesiko iwukuqoqa ama-metabolite ebhaktheriya, futhi ama-metabolites ancibilika kalula emanzini, isiko elihlanekezelwe lingenza kube lula ukuqoqwa.
Ngesikhathi sesiko, kuzoba nomhwamuko omningi esitsheni samasiko, futhi ukufingqa komhwamuko wamanzi esembozweni sesitsha kuzokhiqiza amaconsi amanzi.Uma isidlo samasiko sibekwe endaweni efanele, amaconsi amanzi azohlakaza amakoloni.Ngale ndlela, ikoloni elikhulu lingase lihlakazeke libe amakoloni amaningi amancane, okubangela inkinga enkulu ekutshalweni nokubala kwamagciwane.Uma kubangelwa, i-culture medium iphezulu futhi isidlo singaphansi kwesembozo, futhi amaconsi amanzi ngeke awele ekoloni.
Isikhathi sokuthumela: Sep-20-2022