Ubuchwepheshe bosikompilo lwamaseli ezilwane/ezitshalo kuwuchungechunge lwemisebenzi ye-biotechnology eyenziwa kumaseli ezitshalo angawodwa noma ama-protoplasts ezingeni lamaselula ngenjongo ethile.Kubandakanya ukuzihlukanisa, isiko, ukuvuselelwa kanye nochungechunge lwemisebenzi ehlobene.Mayelana nokukhiqizwa kwezinhlanganisela eziwusizo, kubhekiselwa kakhulu enqubweni yokukhiqiza izinhlanganisela eziwusizo ngokusebenzisa isiko lokumiswa kwamaseli ezitshalo ngaphansi kwezimo eziyinyumba.
Shandong Labioi-serial cell shake flask yenzelwe isiko lamaseli amisiwe angama-293, C HH namanye amaseli.I-shake flask inephansi eliyisicaba kanye nesembozo esiphefumulayo.Ingasetshenziswa ekuthuthukisweni kwezinqubo ezincane, ukukhulisa isinyathelo ngesinyathelo kanye nezinye izigaba zamasiko.Isembozo esiphefumulayo sinefilimu ephefumulayo engu-0.2μm, ephefumulayo futhi engangeni emanzini, engavimbela ngokuphumelelayo ukungena kwama-microorganisms, ukuvimbela ukungcola, nokuqinisekisa ukushintshaniswa kwegesi, okuvumela amaseli ukuba akhule kahle.
Uma usebenzisa i-triangular shake flask, kungcono kakhulu ukulawula inani le-culture medium engezwe ku-20 kuya ku-30% wevolumu yeflask.Kunemigqa ecacile yokuthweswa iziqu kuflask ye-triangular shake ukuze kube lula ukubhekisela kuyo.Ijubane lokuzungezisa le-shaker linconywa ukuthi lilawulwe ku-75 ~ 125RPM, elingalungiswa ngokuya ngesimo sangempela.
Isikhathi sokuthumela: Dec-21-2023