Ochwepheshe bamasiko amaseli avumeleke: Faka Isiko Seseli

Ochwepheshe bamasiko amaseli avumeleke: Faka Isiko Seseli

I-Cell Culture Insert, ebizwa nangokuthi izisekelo ezivunyelekayo, njengoba igama liphakamisa, ukufakwa kwesiko okusetshenziselwa ukuhlolwa okuhlobene nomsebenzi wokungena.Kukhona ulwelwesi olukwazi ukungena ngaphansi kokufakwa kwesiko olunama-micropores osayizi abahlukene.Enye inkomishi yenziwe ngezinto ezifanayo njengepuleti le-orifice elijwayelekile.

Ukufakwa kosikompilo lwamaseli kuvame ukusetshenziswa ekuhlolweni kwamaseli, njengokuhlolwa kwe-co-culture, ukuhlolwa kwe-chemotaxis, ukuhlolwa kokufuduka kwe-tumor cell, ukuhlasela kwe-tumor cell, kanye nokuthuthwa kwamaseli..

Phakathi kwazo, izisekelo ezivunyelekayo zingathuthukisa ngempumelelo isiko lamaseli e-polar ngoba lezi zisekelo zivumela amaseli ukuthi akhiphe futhi amunce ama-molecule endaweni yawo eyisisekelo ne-apical, ngaleyo ndlela agaywe ngendlela engokwemvelo futhi alingise imvelo ye-vivo ukuze enze imigqa ethile yeseli ekhethekile. .

Ngokusho kwamapuleti ahlukene, ukufakwa kwesiko kungahlukaniswa kube ngu-6-umthombo, u-12-umthombo, no-24-well.

Ngokusho kobubanzi obuhlukahlukene bembotshana, ahlukaniswe abe ngu-0.4μm, 3μm, 5μm no-8μm ukusuka kububanzi obuncane bembotshana kuya kububanzi obukhulu bembotshana.

Isici:

• Idizayini yonqenqema emisha yokuhlanganisa isampula elula

• I-PC membrane: izinga eliphansi le-adsorption, linciphisa ukulahlekelwa kwamaprotheni amangqamuzana amancane nezinye izinhlanganisela

• Ifilimu ye-PET inokucaca okuhle kakhulu nokucaca okungcono kokubona, okwenza kube lula ukubona isimo seseli

• Ihambisana nezinyibilikisi eziningi zokulungisa kanye namabala

• Itholakala ngosayizi abahlukahlukene, ingasetshenziswa namapuleti amasiko angu-6-well, 12-well, 24-source-24 kanye nezitsha eziyi-100mm