Izimfuneko zokuqoqwa kwesampula, ukugcinwa nezokuthutha zokuhlola okuvamile

Izimfuneko zokuqoqwa kwesampula, ukugcinwa nezokuthutha zokuhlola okuvamile

1. Ukuqoqwa kanye nokulondolozwa kwezibonelo ze-pathological:

☛Isigaba esiqandisiwe: Susa amabhulokhi ezicubu ezifanele bese uwagcina ku-nitrogen ewuketshezi;

☛Ukuhlukaniswa kukapharafini: Susa amabhulokhi ezicubu ezifanele bese uwagcina ku-4% we-paraformaldehyde;

☛Amaslayidi eseli: Amaslayidi eseli aye alungiswa ku-4% paraformaldehyde imizuzu engu-30, abese eshintshwa nge-PBS futhi acwiliswa ku-PBS futhi agcinwa ku-4°C.

2. Ukuqoqwa nokulondolozwa kwezibonelo zebhayoloji yamangqamuzana:

☛Izicubu ezintsha: Sika isifanekiso bese usigcina ku-nitrogen ewuketshezi noma -80°C esiqandisini;

☛Izifanekiso zikapharafini: Gcina endaweni yokushisa yegumbi;

☛Isampula yegazi eliphelele: Thatha inani elifanele legazi eliphelele bese wengeza i-EDTA noma ishubhu lokuqoqa igazi le-heparin anticoagulation;

☛Amasampula oketshezi lomzimba: i-centrifugation enesivinini esikhulu ukuqoqa inzika;

☛Izibonelo zamaseli: Amaseli ahlanzwa nge-TRizol futhi agcinwe ku-nitrogen ewuketshezi noma -80°C esiqandisini.

3. Ukuqoqwa nokugcinwa kwezibonelo zokuhlolwa kwamaprotheni:

☛Izicubu ezintsha: Sika isifanekiso bese usigcina ku-nitrogen ewuketshezi noma -80°C esiqandisini;

☛Isampula yegazi eliphelele: Thatha inani elifanele legazi eliphelele bese wengeza i-EDTA noma ishubhu lokuqoqa igazi le-heparin anticoagulation;

☛Izibonelo zamaseli: Amaseli ahlanzwa ngokugcwele ngesisombululo se-cell lysis abese egcinwa ku-nitrogen ewuketshezi noma -80°C esiqandisini.

4. Ukuqoqwa nokugcinwa kwe-ELISA, i-radioimmunoassay, nezibonelo zokuhlolwa kwe-biochemical:

☛Isampula ye-Serum (plasma): Thatha igazi lonke ulingeze eshubhuni ye-procoagulation (ishubhu le-anticoagulation), i-centrifuge ku-2500 rpm cishe imizuzu engu-20, qoqa amandla amakhulu, futhi uwagcine ku-nitrogen ewuketshezi noma -80°C esiqandisini;

☛Isampula yomchamo: centrifuge isampula ku-2500 rpm cishe imizuzu engu-20, futhi uyigcine ku-nitrogen ewuketshezi noma -80°C esiqandisini;bhekisela kule ndlela ye-thoracic kanye ne-ascites fluid, i-cerebrospinal fluid, ne-alveolar lavage fluid;

☛Amasampula eseli: Lapho uthola izingxenye ezifihliwe, faka phakathi amasampula ku-2500 rpm cishe imizuzu engu-20 futhi uwagcine ku-nitrogen ewuketshezi noma -80°C esiqandisini;lapho uthola izakhi ze-intracellular, Nciphisa ukumiswa kweseli nge-PBS futhi umise futhi uncibilike ngokuphindaphindiwe ukuze ucekele phansi amaseli futhi ukhulule izingxenye ze-intracellular.I-Centrifuge ku-2500 rpm cishe imizuzu engu-20 bese uqoqa amandla angaphezu kwamandla njengoba ngenhla;

☛Amasampula ezicubu: Ngemva kokusika izibonelo, zikale bese uziqandisa ku-nitrogen ewuketshezi noma -80°C esiqandisini ukuze zisetshenziswe kamuva.

5. Iqoqo lesampula le-Metabolomics:

☛Isampula yomchamo: Faka i-Centrifuge isampula ku-2500 rpm cishe imizuzu engu-20 bese uyigcina ku-nitrogen ewuketshezi noma -80°C esiqandisini;bhekisela kule ndlela ye-thoracic kanye ne-ascites fluid, i-cerebrospinal fluid, i-alveolar lavage fluid, njll;

☛Ngemva kokusika isampula yethishu, yikale bese uyiqandisa ku-nitrogen ewuketshezi noma -80°C esiqandisini ukuze sisetshenziswe kamuva;