Amapuleti amasiko eseli angahlukaniswa abe phansi ayisicaba futhi aphansi ayindilinga (omise okwe-U kanye no-V) ngokuya ngokuma kwephansi;Inani lemigodi yesiko lalingu-6, 12, 24, 48, 96, 384, 1536, njll;Ngokwezinto ezahlukahlukene, kukhona ipuleti leTerasaki kanye nepuleti elijwayelekile lesiko lamaseli.Ukukhetha okukhethekile kuncike ohlotsheni lwamaseli akhulisiwe, umthamo wamasiko odingekayo kanye nezinjongo zokuhlola ezahlukahlukene.
(1) Umehluko nokukhethwa kwamapuleti amasiko aphansi ayisicaba futhi ayindilinga (amise u-U futhi anomumo ongu-V).
Izinhlobo ezahlukene zamapuleti amasiko anokusetshenziswa okuhlukile.Amaseli e-culture ngokuvamile anephansi eliyisicaba, elilungele ukubhekwa nge-microscopic, nendawo ephansi ecacile kanye nezinga likaketshezi lesiko lamaseli elingashintshi.Ngakho-ke, lapho wenza i-MTT nokunye ukuhlola, ipuleti eliphansi eliyisicaba livame ukusetshenziswa, kungakhathaliseki ukuthi amaseli anamathiselwe odongeni noma amisiwe.Ipuleti eliyisicaba eliphansi kufanele lisetshenziswe ukukala inani lokumunca.Naka ngokukhethekile okokusebenza, bese umaka “I-Tissue Culture (TC) Treated” yesiko lamaseli.
Amapuleti amise okwe-U noma amise okwe-V ngokuvamile asetshenziswa kwezinye izidingo ezikhethekile.Isibonelo, ku-immunology, lapho ama-lymphocyte amabili ahlukene exubene nesiko, adinga ukuthintana futhi avuselelane.Ngalesi sikhathi, amapuleti amise okwe-U ngokuvamile asetshenziswa ngoba amaseli azobuthana ebangeni elincane ngenxa yomphumela wamandla adonsela phansi.Ipuleti eliyindilinga eliphansi eliyisiko lingasetshenziselwa ukuhlola ukufakwa kwe-isotopu, okudinga ithuluzi lokuqoqa amaseli ukuze liqoqe isiko lamaseli, njengokuthi “isiko le-lymphocyte elixubile”.Amapuleti amise okwe-V avame ukusetshenziselwa ukubulawa kwamaseli kanye nokuhlolwa kwe-immunological agglutination yegazi.Ukuhlolwa kokubulawa kwamaseli kungathathelwa indawo ipuleti elimise okwe-U (ngemuva kokwengeza amaseli, i-centrifuge ngesivinini esiphansi).
(2) Umehluko phakathi kwepuleti le-Terasaki nepuleti elivamile le-cell culture
Ipuleti leTerasaki lisetshenziselwa ikakhulukazi ucwaningo lwe-crystallographic.Idizayini yomkhiqizo ilungele ukubonwa kwekristalu nokuhlaziywa kwesakhiwo.Kunezindlela ezimbili: ukuhlala nokulenga ukudonsa.Lezi zindlela ezimbili zisebenzisa ukumiswa komkhiqizo okuhlukile.I-Crystal class polymer ikhethwa njengezinto ezibonakalayo, futhi izinto ezikhethekile zilungele ukubuka ukwakheka kwekristalu.
Ipuleti yesiko leseli lenziwe ikakhulukazi ngezinto ze-PS, futhi impahla iphathwa endaweni, elungele ukukhula nokunwetshwa kweseli.Yiqiniso, kukhona nezinto zokukhula zamaseli e-planktonic, kanye nendawo ephansi yokubopha.
(3) Umehluko phakathi kwepuleti le-cell culture ne-Elisa plate
Ipuleti le-Elisa ngokuvamile libiza kakhulu kunepuleti le-cell culture.I-cell plate isetshenziselwa ikakhulukazi isiko lamaseli futhi ingasetshenziswa futhi ukukala ukuhlushwa kwamaprotheni;Ipuleti le-Elisa lihlanganisa ipuleti lokunamathela kanye nepuleti lokuphendula, futhi ngokuvamile alidingi ukusetshenziselwa isiko lamaseli.Isetshenziselwa ikakhulukazi ukutholwa kwamaprotheni ngemuva kokusabela okuxhumene ne-enzyme-immune, edinga izidingo eziphakeme kanye nesixazululo sokusebenza selebula le-enzyme ethile.
(4) Indawo engezansi enembobo kanye nesilinganiso soketshezi esinconyiwe samapuleti amasiko ahlukene asetshenziswa kakhulu
Izinga loketshezi loketshezi oluyisiko elingezwe kumapuleti ahlukene we-orifice akufanele lijule kakhulu, ngokuvamile libe phakathi kwebanga elingu-2~3mm.Inani eliwuketshezi elifanele lembobo yesiko ngalinye lingabalwa ngokuhlanganisa indawo engezansi yezimbobo ezahlukene.Uma uketshezi oluningi lwengezwa, ukushintshaniswa kwegesi (i-oksijeni) kuzothinteka, futhi kulula ukuchichima ngesikhathi senqubo ehambayo, okubangela ukungcola.Ukuminyana kwamaseli athile kuncike enjongweni yokuhlolwa.
Isikhathi sokuthumela: Nov-04-2022