Izinyathelo Eziqondile Zesiko Leseli

1. Imishini evamile

1. Imishini egumbini lokulungiselela

I-distiller yamanzi e-distilled eyodwa, i-double distilled water distiller, ithangi le-asidi, ihhavini, i-pressure cooker, ikhabethe lokugcina (ukugcina izinto ezingahlanzekile), ikhabethe lokugcina (ukugcina i-athikili ehlanzekile), itafula lokupakisha.Imishini egumbini lokulungiselela isixazululo: ibhalansi ye-torsion kanye nebhalansi kagesi (umuthi wokukala), imitha engu-PH (ukulinganisa inani elingu-PH lesisombululo samasiko), i-magnetic stirrer (ilungisa igumbi lesisombululo ukuze linyakaze isixazululo).

2. Izinsiza zegumbi lamasiko

Ithangi le-nitrogen eliwuketshezi, ikhabethe lokugcina (ama-sundries okugcina), ilambu le-fluorescent nesibani se-ultraviolet, uhlelo lokuhlanza umoya, isiqandisi sezinga lokushisa eliphansi (- 80 ℃), isiqandisi somoya, isilinda se-carbon dioxide, ithebula eliseceleni (amarekhodi okuhlola okubhala).

3. Izinto ezisetshenziswayo okumele zibekwe egunjini eliyinyumba

I-Centrifuge (iqoqo lamaseli), i-ultra-clean worktable, i-microscope ehlanekezelwe, i-CO2 incubator (isiko lokufukamela), ukugeza ngamanzi, umshini wokubulala amagciwane ane-oxygen emithathu kanye nomshini wokuvala inzalo, 4 ℃ isiqandisi (ukubeka i-serum nesisombululo sesiko).

2. Ukusebenza kwe-Aseptic

(1) Ukuvalwa inzalo kwegumbi eliyinyumba

1. Hlanza igumbi eliyinyumba njalo: kanye ngesonto, sebenzisa kuqala amanzi okumpompi ukuze ukolobhe phansi, usule itafula, futhi uhlanze itafula lokusebenza, bese usebenzisa i-3 ‰ lysol noma i-bromogeramine noma i-0.5% ye-peracetic acid ukuze usule.

2. Ukwenziwa inzalo kwe-CO2 incubator (i-incubator): qala usule nge-3 ‰ bromogeramine, bese usula ngo-75% wotshwala noma u-0.5% we-peracetic acid, bese u-irradiate ngesibani se-ultraviolet.

3. Ukuvala inzalo ngaphambi kokuhlolwa: vula isibani se-ultraviolet, isibulala-magciwane esinomoya-mpilo emithathu kanye nesistimu yesihlanzi somoya imizuzu engu-20-30 ngokulandelana.

4. Ukuvala inzalo ngemva kokuhlolwa: sula itafula elihlanzekile kakhulu, itafula elihlangothini kanye nesiteji esibona isibonakhulu esihlanekezelwe ngotshwala obungama-75% (3 ‰ bromogeramine).

Ukulungiswa kokuvala inzalo kwabasebenzi baselabhorethri

1. Geza izandla ngensipho.

2. Gqoka izingubo zokuzihlukanisa, amakepisi okuzihlukanisa, izifihla-buso namaslipper.

3. Sula izandla ngebhola likakotini lika-alcohol elingu-75%.

Ukuboniswa komsebenzi oyinyumba

1. Wonke amabhodlela otshwala, i-PBS, i-culture medium kanye ne-trypsin alethwe ebhentshini lokusebenza elihlanzeke kakhulu kufanele kusulwe ngotshwala obungama-75% endaweni engaphandle yebhodlela.

2. Sebenza eduze nelangabi lesibani sotshwala.

3. Izitsha kufanele zihlanzwe inzalo ngaphambi kokusetshenziswa.

4. Izitsha (njengezikepisi zamabhodlela kanye namaconsi) eziqhubeka zisetshenziswa kufanele zibekwe endaweni ephakeme, futhi kufanele zisashise kakhulu ngesikhathi zisetshenziswa.

5. Yonke imisebenzi kufanele ibe seduze nesibani sotshwala, futhi isenzo kufanele sibe lula futhi sinembile, futhi akufanele sithintwe ngokungahleliwe.Uma utshani lungakwazi ukuthinta ithange le-liquid engcolile.

6. Lapho ulangazelela izinhlobo ezingaphezu kwezimbili zoketshezi, naka ukufaka esikhundleni sepayipi elimunca ukuvimbela ukungcoliswa okunqamulayo.

Bheka isahluko esilandelayo ukuze uthole ukukhishwa kwamagciwane kwezinsimbi.