I-Centrifuge tube iwuhlobo lwesiqukathi sesampula esinamashubhu esisebenzisa ubuchwepheshe be-centrifugation ukuze sihlukanise futhi silungise amasampula ebhayoloji.Amandla amakhulu e-centrifugal ahlinzekwa ukujikeleza kwe-centrifuge ngesivinini esikhulu enza izinhlayiya ezincane kuketshezi zihlale futhi zihlukane nesixazululo.Ishubhu ye-centrifuge yenziwe nge-polypropylene yezinto ezibonakalayo zekhwalithi ephezulu, esetshenziswa kabanzi emisebenzini ehlukahlukene yokuhlola futhi ihlangabezana nezidingo zezinga lokuhlaziywa kwebhayoloji.
Ubuchwepheshe be-Centrifuge:
Isetshenziswe kabanzi kusayensi yezinto eziphilayo, ikakhulukazi emkhakheni we-biochemistry kanye ne-molecular biology.Yonke ilabhorethri yebhayoloji ye-biochemical kanye ne-molecular kufanele ilungiselele izinhlobo ezihlukahlukene zama-centrifuge.Ubuchwepheshe be-Centrifuge busetshenziswa kakhulu ekuhlukaniseni nasekulungiseleleni amasampula ezinto eziphilayo ahlukahlukene.Ukumiswa kwesampula yebhayoloji kufakwa kushubhu ye-centrifuge ngaphansi kokuzungezisa ngesivinini esikhulu.Ngenxa yamandla amakhulu e-centrifugal, izinhlayiya ezincane ezimisiwe (njengemvula yama-organelles, ama-macromolecules ezinto eziphilayo, njll.) zihlala ngesivinini esithile, ukuze zihlukaniswe nesixazululo.
I-Centrifuge tube iwuhlobo lwesiqukathi sesampula esinamashubhu esisebenzisa ubuchwepheshe be-centrifugation ukuze sihlukanise futhi silungise amasampula ebhayoloji.Amandla amakhulu e-centrifugal ahlinzekwa ukujikeleza kwe-centrifuge ngesivinini esikhulu enza izinhlayiya ezincane kuketshezi zihlale futhi zihlukane nesixazululo.Ishubhu ye-centrifuge yenziwe nge-polypropylene yezinto ezibonakalayo zekhwalithi ephezulu, esetshenziswa kabanzi emisebenzini ehlukahlukene yokuhlola futhi ihlangabezana nezidingo zezinga lokuhlaziywa kwebhayoloji.
Ubuchwepheshe be-Centrifuge:
Isetshenziswe kabanzi kusayensi yezinto eziphilayo, ikakhulukazi emkhakheni we-biochemistry kanye ne-molecular biology.Yonke ilabhorethri yebhayoloji ye-biochemical kanye ne-molecular kufanele ilungiselele izinhlobo ezihlukahlukene zama-centrifuge.Ubuchwepheshe be-Centrifuge busetshenziswa kakhulu ekuhlukaniseni nasekulungiseleleni amasampula ezinto eziphilayo ahlukahlukene.Ukumiswa kwesampula yebhayoloji kufakwa kushubhu ye-centrifuge ngaphansi kokuzungezisa ngesivinini esikhulu.Ngenxa yamandla amakhulu e-centrifugal, izinhlayiya ezincane ezimisiwe (njengemvula yama-organelles, ama-macromolecules ezinto eziphilayo, njll.) zihlala ngesivinini esithile, ukuze zihlukaniswe nesixazululo.
Isizathu:
Lapho izinhlayiya (i-biological macromolecule noma i-organelle) ingaphansi kwamandla we-centrifugal ngaphansi kokujikeleza kwesivinini esikhulu, amandla e-centrifugal "F" achazwa ngefomula elandelayo, okungukuthi: F=ma=m ω 2 ra - ukusheshisa kokujikeleza kwezinhlayiyana, m - Isisindo esisebenzayo sokumisa izinhlayiyana, ω— Isivinini se-Angular sokujikeleza kwezinhlayiyana, r - i-radius of particle rotation (cm).I-centrifugal force ivamise ukuvezwa yi-multiple of gravity yomhlaba, ngakho-ke ibizwa ngokuthi i-related centrifugal force “RCF”.Noma phindaphinda inombolo ngo-“g” ukumela, isibonelo, 25000 × g.Kusho ukuthi amandla e-centrifugal ahlobene angama-25000.
Isizathu:
Lapho izinhlayiya (i-biological macromolecule noma i-organelle) ingaphansi kwamandla we-centrifugal ngaphansi kokujikeleza kwesivinini esikhulu, amandla e-centrifugal "F" achazwa ngefomula elandelayo, okungukuthi: F=ma=m ω 2 ra - ukusheshisa kokujikeleza kwezinhlayiyana, m - Isisindo esisebenzayo sokumisa izinhlayiyana, ω— Isivinini se-Angular sokujikeleza kwezinhlayiyana, r - i-radius of particle rotation (cm).I-centrifugal force ivamise ukuvezwa yi-multiple of gravity yomhlaba, ngakho-ke ibizwa ngokuthi i-related centrifugal force “RCF”.Noma phindaphinda inombolo ngo-“g” ukumela, isibonelo, 25000 × g.Kusho ukuthi amandla e-centrifugal ahlobene angama-25000.
Izici ze-Labio's centrifugal tube:
1. Ibanga lokubekezelela izinga lokushisa ngu-80~121 ℃, elingasekela izinga lokushisa eliphezulu nokuvala inzalo yokucindezela okukhulu;
2. I-average Zui isivinini sokuhlola esikhulu 8500 RPM (r/min);
3. Ukuhlolwa kokuvuza kwezinga lokushisa eliphezulu: akukho ukuvuza lapho uzungeza ku-65 ℃ amahora angu-2;
4. Udonga lwe-tube lubushelelezi futhi lusobala, olulungele ukusebenza kokuhlolwa nokubhekwa;
5. Ongakukhetha γ Ukuvalwa inzalo ngemisebe.Ukucaciswa kuhlanganisa 1.5ml/2ml micro centrifuge tube kanye 15ml/50ml centrifuge tube.I-Material: I-Polypropylene PP, ihlukaniswe njenge-tapered bottom, ngezansi eyindilinga kanye nezansi ezizisekelayo.
Isikhathi sokuthumela: Oct-19-2022