IELISAipuleti iyithuluzi elibalulekile le-ELISA, i-enzyme-linked immunosorbent assay.Kunezici eziningi ezithinta impumelelo yokuhlolwa kwe-ELISA.Ukukhetha ithuluzi elilungile kuyisinyathelo sokuqala.Ukukhetha i-microplate efanelekile kuzosiza ukuhlola ukuthi kuphumelele.
Impahla yeELISAipuleti ngokuvamile i-polystyrene (PS), futhi i-polystyrene ayinakho ukuzinza kwamakhemikhali futhi ingancibilika ngezinhlobonhlobo zezincibilikizi eziphilayo (njengama-hydrocarbon anephunga elimnandi, ama-halogenated hydrocarbon, njll.), futhi ingagqwala ngama-asidi aqinile nama-alkali.Ayimelani namafutha futhi ishintshe umbala kalula ngemva kokuvezwa ukukhanya kwe-UV.
Yiziphi izinhloboELISAamapuleti akhona?
✦Khetha ngombala
Ipuleti elisobala:ifanele ama-immunoassays amaningi kanye nekhwalithi eqinile kanye nokuhlola okubophayo;
Ipuleti elimhlophe:ilungele i-self-luminescence kanye ne-chemiluminescence;
Ipuleti elimnyama:ilungele ama-immunoassays e-fluorescent kanye nokuhlola okubophayo.
✦Khetha ngokubopha amandla
Ipuleti elibopha kancane:Ibophezela kancane kumaprotheni ngamabhondi e-hydrophobic angaphezulu.Ifaneleka njengesithwali sesigaba esiqinile samaprotheni ama-macromolecular anesisindo se-molecular> 20kD.Umthamo wayo wokubopha amaprotheni ngu-200~300ng IgG/cm2.
Ipuleti elibophezelayo eliphezulu:Ngemva kokwelashwa okungaphezulu, amandla ayo okubopha amaprotheni athuthukiswa kakhulu, afinyelele ku-300 ~ 400ng IgG/cm2, futhi isisindo samangqamuzana amaprotheni aboshwe ngokuyinhloko si>10kD.
✦Hlunga ngesimo esingezansi
Iphansi eliphansi:inkomba ephansi ye-refractive, efanelekile ukutholwa nabafundi be-microplate;
U ngezansi:Inkomba ye-refractive iphezulu, elungele ukungeza, ukufisa, ukuxuba neminye imisebenzi.Ungakwazi ukubona ngokuqondile izinguquko zombala ngokuhlola okubonakalayo ngaphandle kokuyibeka kumfundi we-microplate ukuze unqume ukuthi kukhona yini ukusabela okuhambisana nokuzivikela komzimba.
Isikhathi sokuthumela: Dec-22-2023