Ukusetshenziswa Nezinyathelo Zokuqaphela I-Pipettor

I-pipettor iyithuluzi laselabhorethri elivame ukusetshenziswa elisetshenziselwa ukudluliswa okunembile koketshezi.Iqukethe ikhanda lesibhamu, umbhobho wesibhamu, irula, inkinobho nezinye izingxenye.Inezinzuzo zokusebenza okulula nokunemba okuphezulu, futhi isetshenziswa kabanzi ku-biology, chemistry, imithi kanye neminye imikhakha.Lesi sihloko sizokwethula inhloso, ukusetshenziswa, izinyathelo, ukugcinwa nokugcinwa kwe-pipettor.

1) Inhloso ye-pipettor

I-Pipettor isetshenziswa kakhulu ukudlulisa uketshezi ngokunembile, njengama-buffer, ama-reagents, njll. Ingakhetha amakhanda okumunca ahlukene kanye namandla kuye ngezidingo ukuze kuzuzwe ukudluliswa kwamavolumu ahlukene nezinhlobo ezahlukene zoketshezi.Uma kuqhathaniswa namapayipi endabuko, izibhamu ze-pipette zinezinzuzo zokusebenza okulula, isivinini esisheshayo, nokunemba okuphezulu, okuthuthukisa kakhulu ukusebenza kahle kwelabhorethri.

2) Indlela yokusebenzisa i-pipettor

• Khetha amathiphu alungile

Khetha ithiphu enomthamo ofanele ngokusekelwe ohlotsheni nomthamo woketshezi oludingayo ukuze uludlulise.Ngokuvamile, uhla lokulinganisa lwesibhamu se-pipette luphawulwe emzimbeni wesibhamu, futhi udinga ukukhetha ngokusho kokumaka uma usebenzisa.

• Lungiselela uketshezi

Thela uketshezi oluzodluliselwa esitsheni esihambisanayo, njengethangi le-pipette, ukuze usebenze kalula.

• Setha umthamo

Ungakwazi ukuguqula ngokuqondile inkinobho ukuze ulungise njengoba kudingeka.

• Imbibe

Okokuqala cindezela inkinobho endaweni yokuqala, bese ufaka ithiphu ye-pipette esixazululweni, bese ukhulula kancane inkinobho ukuze uhogele uketshezi.Phakathi nenqubo ye-aspiration, ukunakekelwa kufanele kuthathwe ukuze kugwenywe ithiphu elithinta phansi noma udonga oluhlangothini lwesitsha, futhi i-pipette akufanele ihlotshiswe phansi ngemuva kokufisa.

• Khama

Faka ithiphu esitsheni esiqondiwe, cindezela inkinobho uye endaweni yesibili, bese ukhipha uketshezi.

3) Izinyathelo zokuphepha zokusebenzisa i-pipettor

• Ngaphambi kokusebenzisa, udinga ukufunda imiyalo nezinqubo zokusebenza ngokucophelela ukuze uqonde ukusetshenziswa nezinyathelo zokuphepha.
• Phakathi nenqubo yokudlulisa uketshezi, ithiphu kufanele ivinjwe ukuthi ingathintani nodonga olungezansi noma oluseceleni lwesitsha ukuze kugwenywe ukungcoliswa.
• Uma ulungisa ivolumu, udinga ukulungisa kancane futhi ugweme ukuguqula irula ngokushesha ukuze ugweme ukulimaza i-pipette.
• Ngesikhathi sokusetshenziswa, ukunakekelwa kufanele kuthathwe ukuze kugwenywe ukuchaphazeka koketshezi ukuze kugwenywe ukungcoliswa kwemvelo kanye nezingozi zokuhlola.
• Ngemuva kokusetshenziswa, isibhamu se-pipette sidinga ukuguqulwa sibuyele ebangeni eliphezulu ukuze kugwemeke ukuthi intwasahlobo ibe sesimweni esivumelwaneni isikhathi eside futhi ithinte ukunemba kwesibhamu se-pipette.

4) Ukunakekelwa nokugcinwa kwe-Pipettor

• Hlanza ithiphu lesibhamu.Ngemuva kokusetshenziswa, ikhanda lesibhamu lidinga ukuhlanzwa ukuze kuvinjelwe izinsalela ekungcoliseni ukuhlolwa okulandelayo.Lapho uhlanza, kufanele kuqashelwe ukugwema ukulimaza izingxenye ngaphakathi kwesibhamu.
• Hlola izinkinobho nerula.Ngesikhathi sokusetshenziswa, udinga ukuhlola njalo ukuthi izinkinobho namarula akhululekile noma ayawa.Uma kukhona okungahambi kahle, kumele kulungiswe noma kushintshwe ngesikhathi.
• Ukugcinwa njalo.Yenza ukunakekelwa okuvamile ku-pipette, kuhlanganise nokugcinwa kwezingxenye zangaphakathi, ukushintshwa kwezimpawu, njll, ukuze kuqinisekiswe ukusebenza kwayo okujwayelekile nokunemba.
• Isitoreji.Gcina i-pipette endaweni eyomile, engenalo uthuli futhi ugweme ukuchayeka isikhathi eside emoyeni ukuze ugweme ukugqwala nokungcola.

Ngaphezu kwalokho, i-pipettor kagesi isetshenziswa kabanzi kumalabhorethri ukuze imunce, idlulise, futhi ixube uketshezi ngenxa yezinzuzo zabo ezisheshayo, ezisebenzayo nezilula.Ngokuvamile zisetshenziswa ngamapayipi epulasitiki alahlwayo ngesikhathi sokusetshenziswa.

Ngamafuphi, ukusetshenziswa okulungile nokugcinwa kwe-pipettor kuyisiqinisekiso esibalulekile sokuqinisekisa ikhwalithi nokuphepha komsebenzi welabhorethri.Ngesikhathi sokusetshenziswa, kuyadingeka ukuthobela ngokuqinile izinqubo zokusebenza nezinyathelo zokuphepha, futhi wenze ukugcinwa nokugcinwa njalo ukuze kuqinisekiswe ukusebenza kwayo okujwayelekile nokunemba.