Amathiphu ekukhetheni nasekusetshenzisweni kwamapuleti e-cell culture (I)
Njengethuluzi elivamile nelibalulekile lesiko lamaseli, ipuleti yesiko lamaseli linobumo obuhlukahlukene, imininingwane kanye nokusetshenziswa.
Ingabe nawe udidekile mayelana nendlela yokukhetha ipuleti lesiko elilungile?
Ingabe ukhathazekile ngokuthi ungasebenzisa kanjani ipuleti lesiko ngendlela efanele?
Ingabe udidekile mayelana nendlela yokubhekana nepuleti lesiko?
Uzizwa kanjani ngokusetshenziswa okumangalisayo kwepuleti yesiko elihlukile?
Ungakhetha kanjani ipuleti le-cell culture?
1) Amapuleti amasiko eseli angahlukaniswa abe ngezansi futhi aphansi ayisicaba (omise okwe-U kanye no-V) ngokuya ngokuma kwephansi;
2) Inani lemigodi yesiko lalingu-6, 12, 24, 48, 96, 384, 1536, njll;
3) Ngokwezinto ezahlukahlukene, kukhona ipuleti leTerasaki kanye nepuleti elijwayelekile lesiko lamaseli.Ukukhetha okukhethekile kuncike ohlotsheni lwamaseli akhulisiwe, umthamo wamasiko odingekayo kanye nezinjongo zokuhlola ezahlukahlukene.
Umehluko kanye nokukhethwa kwamapuleti amasiko aphansi ayisicaba futhi ayindilinga (amise oku-U kanye no-V).
Izinhlobo ezahlukene zamabhodi ngokwemvelo zinokusetshenziswa okuhlukile
Zonke izinhlobo zamaseli aphansi ayisicaba zingasetshenziswa, kodwa uma inani lamaseli lilincane, njenge-cloning, kusetshenziswa amapuleti aphansi angama-96 aphansi.
Ngaphezu kwalokho, lapho wenza i-MTT nokunye ukuhlola, ipuleti eliphansi eliyisicaba ngokuvamile lisetshenziselwa kokubili amaseli anamathelayo namaseli amisiwe.
Ngokuqondene namapuleti amise okwe-U noma amise okwe-V, ngokuvamile asetshenziswa kwezinye izidingo ezikhethekile.Isibonelo, ku-immunology, lapho ama-lymphocyte amabili ahlukene exubene, adinga ukuxhumana ukuze avuselele.Ngakho-ke, amapuleti amise okwe-U ngokuvamile ayadingeka.Ngenxa yokuthi amaseli azohlangana ebangeni elincane ngenxa yomphumela wamandla adonsela phansi, amapuleti amise okwe-V awasebenziseki kangako.Amapuleti amise okwe-V ngokuvamile asetshenziswa ekuhloleni kokubulawa kwamaseli ukuze kwenziwe amaseli okuqondiwe asondele eduze, kodwa amapuleti amise okwe-U angasetshenziswa futhi kulokhu kuhlolwa (ngemuva kokwengeza amaseli, i-centrifuge ngesivinini esiphansi).
Uma isetshenziselwa isiko lamaseli, ngokuvamile iphansi phansi.Ngaphezu kwalokho, ukunakekelwa okukhethekile kufanele kukhokhwe endabeni.Uphawu oluthi “Tissue Culture (TC) Treated” lusetshenziselwa isiko lamaseli.
Iphansi eliyindilinga livamise ukusetshenziselwa ukuhlaziya, ukusabela kwamakhemikhali, noma ukulondolozwa kwesampula.Ngoba izingezansi eziyindilinga zingcono ukumunca uketshezi, kanti ezansi eziyisicaba azikho.Nokho, uma ufuna ukukala inani lokumunca ukukhanya, kufanele uthenge eliphansi eliyisicaba.
Amasiko amaseli amaningi asebenzisa amapuleti aphansi ayisicaba, okulula ukuwabona ngaphansi kwesibonakhulu, anendawo ephansi ecacile, anobude obungaguquki bezinga loketshezi lwamaseli, futhi asiza nokutholwa kwe-MTT.
Ipuleti eliyindilinga eliphansi eliyisiko lisetshenziselwa ikakhulukazi ukuhlola ukufakwa kwe-isotope, futhi ithuluzi lokuqoqa amaseli liyadingeka ukuze kuqoqwe isiko lamaseli, njengokuthi “isiko le-lymphocyte elixubile”.
Isikhathi sokuthumela: Dec-08-2022