Sebenzisa Indlela kanye Nezixwayiso Zeshubhu Lokuqandisa
Ezivivinyweni ze-microbiological, umshini wokuhlola owodwa uvame ukusetshenziswa, okungukuthi, i-cryopreservation tube.Nokho, ngenxa yobunkimbinkimbi bazo obuhlukene, imiphumela ihluka kakhulu.Ngenxa yalesi sizathu, okwamanje, ama-laboratories amaningi e-China enza amashubhu okulondoloza amagciwane ngokwawo, angagcini nje ukwandisa ukuqina komsebenzi, kodwa futhi ngenxa yokulinganiselwa kwezimo ezihlukahlukene, umphumela wokugcinwa kwamagciwane awugculisi ngaso sonke isikhathi.
Ngakho-ke, kuyadingeka ukwazi kahle indlela yokusebenzisa kanye nezinyathelo ezithile zokuphepha ze-cryopreservation tube, ukuze udlale indima enkulu.
1.Indlela yokufaka isicelo
1).Uma usebenzisa ishubhu le-cryopreservation ukugcina amasampula, kuyadingeka ngokuqinile ukufaka ishubhu le-cryopreservation ongqimbeni lomhwamuko we-nitrogen ewuketshezi noma esiqandisini ukuze ugcinwe.Uma ishubhu ye-cryopreservation igcinwe ku-nitrogen eyi-liquid, kunethuba elithile lokuthi i-nitrogen eyi-liquid izongena eshubhu le-cryopreservation.Ngesikhathi sokululama, i-gasification ye-nitrogen ewuketshezi izoholela ekungalinganini kokucindezela kwangaphakathi nangaphandle, okungenzeka kakhulu kubangele i-cryopreservation tube ukuba iqhume, futhi ibe nezingozi zebhayoloji.
2).Sebenzisa ishubhu ye-cryopreservation ukuze uvuselele, futhi usebenzise okokusebenza kokuvikela ukuphepha kuyo yonke inqubo.Kunconywa ukugqoka izingubo zaselabhorethri, amagilavu kakotini futhi usebenze ebhentshini eliphephile laselabhorethri.Uma kungenzeka, sicela ugqoke izibuko noma isivikelo sobuso.Njengoba izinga lokushisa lasendlini ehlobo lizoba phezulu kunalelo lasebusika, sicela uqaphele.
3).Ngesikhathi sokugcinwa kwamaseli e-cryopreserved, izinga lokushisa eliqandayo lamashubhu e-cryopreserved kumele lifane.Ukubanda okungalingani kuzoholela ekujameni kweqhwa, okuzovimbela ukudluliswa kwezinga lokushisa eliwuketshezi nhlangothi zombili, ngaleyo ndlela kudale umfutho ophakeme oyingozi futhi kubangele ukulimala kweshubhu eliqandayo.
4).Inani lamasampuli aqandisiwe akumele leqe umthamo omkhulu wokusebenza odingwa yishubhu eqandisiwe.
2. Izindaba ezidinga ukunakwa
1).Indawo yokugcina amashubhu eqandayo
Amashubhu e-cryopreservation angagcinwa angagcinwa ekamelweni lokushisa noma 2-8 ℃ izinyanga ezingu-12;Ishubhu ye-cryopreservation ene-inoculated ingagcinwa - ku-20 ℃ futhi ibe nomthelela omuhle wokulondoloza ubunzima phakathi nezinyanga eziyi-12;Ishubhu ye-cryopreservation ene-inoculated ingagcinwa ku--80 ℃, futhi uhlobo lungagcinwa kahle phakathi nezinyanga ezingu-24.
2).Isikhathi sokugcina ishubhu eqandayo
Amashubhu e-cryopreservation angasetshenzisiwe angagcinwa ekamelweni lokushisa noma 2-8 ℃;Ishubhu ye-cryopreservation ene-inoculated izogcinwa - 20 ℃ noma - 80 ℃.
3).Izinyathelo zokusebenza ze-tube yokuqandisa
Thatha amasiko amasha avela kumasiko ahlanzekile webhaktheriya ukuze ulungiselele ukumiswa kwebhaktheriya nge-turbidity engaba ngu-3-4 we-McDonnell's ratio yokujova kanye neshubhu lokulondoloza uhlobo;Qinisa ishubhu lokulondoloza bese ulibuyisela emuva naphambili izikhathi ezingu-4-5 ukuze wenze amabhaktheriya afakwe emulsified, ngaphandle kokujikeleza;Faka ishubhu lokulondoloza esiqandisini ukuze ulondolozwe (- 20 ℃ - 70 ℃
Isikhathi sokuthumela: Nov-25-2022