Kufanele sinake le mininingwane lapho senza isiko lamaseli

Ukulungiselela ngaphambi kwesiko lamaseli

Ngaphambi kokuthi ugqoke amagilavu ​​ukuze uqale isiko lamaseli, hlola ukuthi inani lamapayipi namabhodlela lanele yini, ukuze ugweme ukungena nokuphuma kukhonsoli futhi ngemva kokuhlolwa, okunganciphisa ingozi yokungcoliswa kwamaseli.

I-cellculture medium kufanele futhi ishiswe kuqala.Ukukhetha ukushisa kuqala ingxenye ye-medium kunebhodlela lonke ngeke nje ugcine isikhathi sokuhlola, kodwa futhi ugweme ukuwohloka kwamaprotheni okubangelwa ukushisa okuphindaphindiwe okuphakathi.

Ngemuva kokuhlinzwa, ungakhohlwa ukuthi okuphakathi kuyazwela ekukhanyeni futhi kufanele kugcinwe kude nokukhanya ngangokunokwenzeka.
Ukuhlolwa ngezikhathi ezithile kwesiko lamaseli

Ukuhlolwa okuvamile kwe-morphology yamaseli akhulisiwe, okungukuthi, ukuma nokubukeka, kubalulekile ekuphumeleleni kokuhlolwa kwesiko lamaseli.
Ngaphezu kokuqinisekisa isimo esinempilo samangqamuzana, ukuhlola amangqamuzana ngamehlo anqunu kanye nesibonakhulu ngaso sonke isikhathi lapho usebenzisa amangqamuzana nakho kungathola izimpawu zokungcola kusenesikhathi, ukuze kugwenywe ukusabalala kokungcola kwamanye amaseli elabhorethri.
Izimpawu zokuwohloka kwamaseli

Izimpawu zokuwohloka kwamangqamuzana zihlanganisa ukubonakala kwama-granules azungeze i-nucleus, ukuhlukaniswa kwamaseli ku-matrix, nokwakheka kwama-vacuoles e-cytoplasmic.

Lezi zimpawu ze-metamorphic zingabangelwa izizathu eziningi, njenge:

Ukungcoliswa kwesiko, ukuqina komugqa wamaseli, noma ukuba khona kwezinto ezinobuthi endaweni yamasiko, noma lezi zimpawu zibonisa kuphela ukuthi isiko lidinga ukushintshwa.
Uma i-metamorphism ibucayi, izoba yinguquko engenakuhlehliswa.

Ukubulala amagciwane kanye nesakhiwo se-cell culture fume hood

Gcina i-cell culture fume hood ihlanzekile futhi ihlelekile, futhi ubeke zonke izinto phakathi kwebanga lokubuka eliqondile.

Fafaza i-ethanol engu-70% kuzo zonke izihloko ezifakwe kuhood yomusi, usule futhi uzihlanze ukuze zibulawe amagciwane.

Beka isitsha se-cell culture endaweni evulekile phakathi nendawo ye-fume hood;I-pipette ibekwe ngaphambili kwesokudla ukuze kufinyeleleke kalula;I-reagent kanye ne-culture medium ibekwe ngakwesokudla emuva ukuze imunce kalula;I-tube rack yokuhlola ihlelwe engxenyeni engemuva ephakathi;Isitsha esincane sibekwe ngemuva kwesobunxele ukuze sibambe uketshezi olulahlwayo.