I-PCR 8-strip centrifuge isebenzisa amandla amakhulu e-centrifugal akhiqizwa ukuzungezisa ngesivinini esikhulu kwerotha eyi-centrifuge ukuze kuthuthukiswe isivinini sokuxazulula sezinhlayiya kuketshezi nezinto ezihlukene ezinezilinganiso ezihlukene zokuxazulula kanye nokuvuvukala kusampula.Ocwaningweni lwethu lwebhayoloji, sivamise ukusebenzisa i-PCR 8-strip.Imiphi imininingwane okufanele inakwe lapho kusetshenziswa i-PCR 8-strip ?Okulandelayo, i-Labio biological izoyichaza.
1. Ungasebenzisi ngaphandle kokuqeqeshwa nokuhlola.
2. Khetha i-rotor nesivinini esifanele.Noma, ungasebenzisi.
3. Khetha isincibilikisi esiphilayo esifanelekile ngaphezu kwezinga lokushisa (ngokuvamile 4 ℃) ukuze uvimbele ukulimala okungamaqhwa
1. Ungasebenzisi ngaphandle kokuqeqeshwa nokuhlola.
2. Khetha i-rotor nesivinini esifanele.Ungasebenzisi.
3. Khetha isincibilikisi esiphilayo esifanelekile ngaphezu kwezinga lokushisa (ngokuvamile 4 ℃) ukuze uvimbele ukulimala okungamaqhwa
1. Ungasebenzisi ngaphandle kokuqeqeshwa nokuhlola.
2. Khetha i-rotor nesivinini esifanele.Ungasebenzisi.
3. Khetha isincibilikisi esiphilayo esifanelekile ngaphezu kwezinga lokushisa (ngokuvamile ngu-4 ℃) ukuze uvimbele ukulimala okuqandayo kwe-PCR 8-strip kanye nerotha.
4. Ngaphambi kokusebenzisa i-rotor, sula ipayipi ngenduku yembobo bese uhlola ngokucophelela imbobo phansi ukuze uthole imifantu noma amabala amhlophe.Uma kunjalo, bheka emuva bese uyilahla.
5. Inani lesixazululo ku-PCR 8-strip kufanele lifanele.Ipayipi lensimbi engagqwali ayinaso isembozo, i-2/3 kuphela inganamathiselwa.Ishubhu lepulasitiki lingahambisana nehlombe.Ikhava kumele ivalwe ngaphandle kokuvuza.Ukusetshenziswa kwama-solvents e-organic kumapayipi epulasitiki kumele kuhambisane nemithethonqubo.
6. Ama-Centrifuges kufanele abekwe ngababili futhi alinganisele kahle ngokuchezuka<0.1g.
7. Awukwazi ukuzungezisa ngaphandle kokuzungezisa.Ukuphatha kanye ne-rotor, udinga ukuthi sisebenzise isibambo ukuvimbela i-rotor ukuthi ingasheleli.Vula isibani bese usivumela ukuthi sizinze.Uma uphendula isibambo, bamba isibambo ngesandla bese uphendula isibambo.Ikhava yekepisi ejikelezayo kufanele ivalwe ngokuqinile.Ikepisi akufanele libe phakathi nendawo.
8. Ungasivuli isivalo ngesikhathi se-centrifugation.I-Centrifuge ayisekelwe.
9. Ngemuva kokuthi i-rotor isetshenziswe, i-rotor izosuswa ngesikhathi, yomiswe emgodini we-rotor, futhi imigodi embhobho kufanele ihlanzwe ngokucophelela ngenduku yokuhlanza.Uma isisombululo sichichima, sigeze sihlanzeke, sula amanzi ahlanganisiwe emgodini we-rotor, bese uvula umnyango ukuze umise i-rotor cavity.
10. I-centrifuge kufanele igcinwe kahle futhi ibhaliswe kanye nama-manifolds ayisishiyagalombili ngemva kokusetshenziswa.
kanye ne-rotor.
4. Ngaphambi kokusebenzisa i-rotor, sula ipayipi ngenduku yembobo bese uhlola ngokucophelela imbobo phansi ukuze uthole imifantu noma amabala amhlophe.Uma kunjalo, bheka emuva bese uyilahla.
5. Inani lesixazululo ku-octuple kufanele lifanele.Ipayipi lensimbi engagqwali ayinaso isembozo, i-2/3 kuphela inganamathiselwa.Ishubhu lepulasitiki lingahambisana nehlombe.Ikhava kumele ivalwe ngaphandle kokuvuza.Ukusetshenziswa kwama-solvents e-organic kumapayipi epulasitiki kumele kuhambisane nemithethonqubo.
6. Ama-Centrifuges kufanele abekwe ngababili futhi alinganisele kahle ngokuchezuka<0.1g.
7. Awukwazi ukuzungezisa ngaphandle kokuzungezisa.Ukuphatha kanye ne-rotor, udinga ukuthi sisebenzise isibambo ukuvimbela i-rotor ukuthi ingasheleli.Vula isibani bese usivumela ukuthi sizinze.Uma uphendula isibambo, bamba isibambo ngesandla bese uphendula ikhanda.Isembozo sekhanda esiphendukayo kufanele simbozwe ngokuqinile.Isivalo akufanele sibe phakathi nendawo.
8. Ungasivuli isivalo ngesikhathi se-centrifugation.I-Centrifuge ayisekelwe.
9. Ngemuva kokuthi i-rotor isetshenziswe, i-rotor izosuswa ngesikhathi, yomiswe emgodini we-rotor, futhi imigodi embhobho kufanele ihlanzwe ngokucophelela ngenduku yokuhlanza.Uma isisombululo sichichima, sigeze sihlanzeke, sula amanzi ahlanganisiwe emgodini we-rotor, bese uvula umnyango ukuze umise i-rotor cavity.
10. I-centrifuge kufanele igcinwe kahle futhi ibhaliswe kanye nama-manifolds ayisishiyagalombili ngemva kokusetshenziswa.
kanye ne-rotor.
4. Ngaphambi kokusebenzisa i-rotor, sula ipayipi ngenduku yembobo bese uhlola ngokucophelela imbobo phansi ukuze uthole imifantu noma amabala amhlophe.Uma kunjalo, bheka emuva bese uyilahla.
5. Inani lesixazululo ku-octuple kufanele lifanele.Ipayipi lensimbi engagqwali ayinaso isembozo, i-2/3 kuphela inganamathiselwa.Ishubhu lepulasitiki lingahambisana nehlombe.Ikhava kumele ivalwe ngaphandle kokuvuza.Ukusetshenziswa kwama-solvents e-organic kumapayipi epulasitiki kumele kuhambisane nemithethonqubo.
6. Ama-Centrifuges kufanele abekwe ngababili futhi alinganisele kahle ngokuchezuka<0.1g.
7. Awukwazi ukuzungezisa ngaphandle kokuzungezisa.Ukuphatha kanye ne-rotor, udinga ukuthi sisebenzise isibambo ukuvimbela i-rotor ukuthi ingasheleli.Vula isibani bese usivumela ukuthi sizinze.Uma uphendula isibambo, bamba isibambo ngesandla bese uphendula ikhanda.Isembozo sekhanda esiphendukayo kufanele simbozwe ngokuqinile.Isivalo akufanele sibe phakathi nendawo.
8. Ungasivuli isivalo ngesikhathi se-centrifugation.I-Centrifuge ayisekelwe.
9. Ngemuva kokuthi i-rotor isetshenziswe, i-rotor izosuswa ngesikhathi, yomiswe emgodini we-rotor, futhi imigodi embhobho kufanele ihlanzwe ngokucophelela ngenduku yokuhlanza.Uma isisombululo sichichima, sigeze sihlanzeke, sula amanzi ahlanganisiwe emgodini we-rotor, bese uvula umnyango ukuze umise i-rotor cavity.
10. I-centrifuge kufanele igcinwe kahle futhi ibhaliswe kanye ne-PCR 8-strip ngemva kokusetshenziswa.
Isikhathi sokuthumela: Sep-14-2022