Kungani ama-cryovial aqhuma?Ungakugwema kanjani?

Kungani ama-cryovial aqhuma?Ungakugwema kanjani?

Ngesikhathi sokuhlolwa, singase sisebenziseama-cryovialsukufriza amasampula, kodwa lapho kuqhwa nge-nitrogen ewuketshezi,ama-cryovialskuvame ukuqhuma, okungabangeli kuphela ukulahleka kwamasampula okuhlola, kodwa kungase kubangele nomonakalo kumasampuli.Abahloli babangela ukulimala, ngakho-ke ungakuvimbela kanjani lokhu ukuthi kungenzeki?

Izizathu:

Okokuqala nje,ama-cryovialsayikwazi ukubekwa ngokuqondile esigabeni se-liquid nitrogen ukuze kulondolozwe.Ngoba impahla umzimba tube kanye cap of the commonama-cryovialsahlukene, amazinga okwanda okushisayo kanye nokufinyela ngesikhathi sokuqandisa nawo ahlukile.Uma ubeka ii-cryovialngqo esigabeni se-liquid, ungavumela i-nitrogen ewuketshezi ukuthi igeleze eshubhu.Lapho uvuselela isampula ngokuzayo, faka iizitsha ze-cryogenicemanzini okugeza angu-37°C, i-nitrogen eyiketshezi eshubhuni yahwamuka ngokushesha futhi yanwetshwa, kodwa igesi ayikwazanga ukuphunyuka eshubhuni ngesikhathi, okwabangela ukuqhuma kweshubhu le-cryopreservation.

Indlela yokugwema:

1. Ungagciniama-cryovialsngqo esigabeni se-liquid, kodwa esigabeni segesi.Noma vele uyiqhwaze ngqo esiqandisini.Khumbula ukuthi ungayibeki ngqo ngaphansi kobuso be-nitrogen ewuketshezi.

2. Sebenzisa ukuzungezisa kwangaphakathiama-cryotubes.

Yiqiniso, ngisho nangaphakathi liphendukaama-cryotubesayikwazi ukufakwa ngqo esigabeni se-liquid, kodwa ijikelezwe ngaphakathiama-cryotubesbanokubekezelela izinga lokushisa eliphansi kangcono kunamakepisi azungeziswa ngaphandle, anganciphisa amathuba okuqhuma futhi aphephe ngokuqhathaniswa.Ukuzungezisa kwangaphandlei-crystalempeleni yakhelwe ukuqandisa ngomshini, ngakho ayifanele ukugcinwa kwe-nitrogen ewuketshezi.

3. Ngakho-ke yini okufanele uyenze uma udinga ngempela ukuyibeka esigabeni samanzi?Ukusabela kule nkinga, empeleni kunemikhono yamashubhu e-cryopreservation eklanyelwe le njongo ngokukhethekile, engasetshenziswa ukuvala ishubhu le-cryopreservation bese liyibeka esigabeni soketshezi.Yiqiniso, ungasebenzisa futhi ifilimu yokuvala uphawu, i-tape yezokwelapha, njll ukuze uyibeke uphawu, ukuze kungabi khona ukuqhuma.