Kungani ukwelashwa kwe-TC surface kuyadingeka ezintweni ezisebenzisekayo zesiko lamaseli?
Kunezinhlobo ezahlukene zamangqamuzana, angahlukaniswa abe amaseli anamathelayo kanye namaseli amisiwe ngokwezindlela zesiko Amaseli amisiwe amangqamuzana akhula ngokuzimela ngaphandle kokusekelwa, futhi akhule ngokumiswa endaweni yesiko, njengama-lymphocyte Amaseli Anamathelayo. amaseli abambelelayo, okusho ukuthi ukukhula kwamaseli kumele kube nendawo yokusekela enamathelayo.Bangakwazi ukukhula futhi bakhiqize kule ndawo kuphela ngokuncika ezintweni zokunamathela ezifihliwe ngokwazo noma ezihlinzekwe endaweni yamasiko.Amangqamuzana amaningi ezilwane angawamangqamuzana anamathelayo.
Ngaphambilini, izinto eziningi ezisetshenziswayo zesiko lamaseli emakethe zenziwe ngengilazi, okwakuyi-hydrophilic, ngakho-ke indawo engaphezulu yayingadingi ukwelashwa okukhethekile Kodwa-ke, ekusetshenzisweni kwangempela kwenqubo, kukhona ukushiyeka okufana nokungcola futhi kulula ukungcolisa isampula Nge. ukuthuthukiswa okusheshayo kwesayensi nobuchwepheshe, izinto ezihlukahlukene ze-polymer (njenge-polystyrene PS) kancane kancane ziye zashintsha izinto zengilazi futhi zaba izinto eziyisisekelo zokucubungula izinto ezisetshenziswayo zesiko lamaseli.
I-Polystyrene iyi-polymer engahleliwe e-amorphous enokucaca.Imikhiqizo yayo inokucaca okuphezulu kakhulu, nokudluliswa okungaphezu kuka-90%, okulungele ukubheka isimo se-cell culture ngaphansi kwe-microscope.Ngaphezu kwalokho, inezinzuzo zombala olula, uketshezi oluhle lokucubungula, ukuqina okuhle kanye nokumelana nokugqwala kwamakhemikhali okuhle Nokho, ubuso be-polystyrene bu-hydrophobic.Ukuze kuqinisekiswe ukuthi amaseli anamathelayo angakwazi ukunamathisela kahle endaweni yezinto ezisetshenziswayo, ingaphezulu lezinto ezisetshenziswayo zesiko lamaseli lidinga ukuphathwa ngokulungiswa okukhethekile.Izinto ze-hydrophilic zethulwa phezulu ukuze zivumelane nokukhula nokukhiqizwa kwamangqamuzana anamathelayo.Lokhu kwelashwa kubizwa ngokuthi i-TC surface treatment TC treatment isetshenziswa ezitsheni ze-cell culture, amapuleti e-cell culture, amapuleti akhuphuka amaseli, amabhodlela e-cell culture, njll Ngokuvamile, okokusebenza kokwelapha okungaphezulu kwe-plasma kusetshenziselwa ukufeza ukwelashwa okungaphezulu kwe-TC kwezitsha zamaseli.
Izici zesitsha se-cell culture emva kokwelashwa kwe-TC surface
1. Ukuhlanza ngaphambili indawo yomkhiqizo: I-plasma ye-O2 ingakwazi ukumunca izinhlayiya ezincane nokunye ukungcola okunamathiselwe endaweni yomkhiqizo, futhi idonsa igesi exubile iphume egumbini le-vacuum ngepompo ye-vacuum ukuze ifinyelele umphumela wokuhlanza ngaphambili.
2. Yehlisa ukushuba kwesimo somkhiqizo, ukuze i-engeli yokuthinta amanzi yomkhiqizo inciphe kakhulu, futhi ihambisane namandla e-ionization afanelekile nokugxila, ukuze i-angle yokuthinta amanzi yomkhiqizo ongaphezulu WCA<10 °.I-3 I-plasma ye-O2 izosabela ngamakhemikhali endaweni yomkhiqizo, futhi amaqembu amaningi asebenzayo angangezwa endaweni yomkhiqizo, kuhlanganise ne-hydroxyl (- OH), i-carboxyl (- COOH), i-carbonyl (- CO -), i-hydroperoxy (- OOH), njll. Lawa maqembu asebenzayo angathuthukisa isivinini samasiko nomsebenzi phakathi nesiko lamaseli.
Isikhathi sokuthumela: Jan-13-2023